Single nucleotide polymorphisms of taste genes and caries: a systematic review and meta-analysis.

Objective: The aim of the present study was to systematically review the literature investigating the single nucleotide polymorphisms (SNP) related to taste genes and their influence on caries.

Material And Methods: Search was performed in five databases to respond to the question: 'Are the polymorphisms of taste genes associated with dental caries?'. Studies in humans were included.

Genes in the pathway of tooth mineral tissues and dental caries risk: a systematic review and meta-analysis.

Objectives: To perform a systematic review of the literature, investigating the influence of tooth mineral tissues genes on dental caries.

Materials And Methods: Five databases were searched. Only human studies with cross-sectional, longitudinal, and case-control design were included.

Bone, Periodontal and Dental Pulp Regeneration in Dentistry: A Systematic Scoping Review.

The aim of presented systematic scoping review was to investigate the actual and future clinical possibilities of regenerative therapies and their ability to regenerate bone, periodontal and pulp with histological confirmation of the nature of formed tissue. Electronic search was conducted using a combination between Keywords and MeSH terms in PubMed, Scopus, ISI-Web of Science and Cochrane library databases up to January 2016. Two reviewers conducted independently the papers judgment.

Is the use of Cannabis associated with periodontitis? A systematic review and meta-analysis.

Recent studies have shown that there is also biological plausibility for a possible relationship between periodontal disease and Cannabis use, thus the aim of this study was to investigate whether the use of Cannabis is associated with periodontitis. Electronic searches were performed in PubMed, Scopus, ISI-Web of Science, BVS-Virtual health library and Scielo without restrictions. Search strategy was performed using relevant keywords considering the structure of each database.

Effect of temperature and storage time on dental bleaching effectiveness.

Objective: To evaluate the effect of storage time and temperature on the effectiveness of bleaching agents.

Methods: Enamel slabs were randomly allocated according to the concentration of carbamide peroxide (CP): 10% (CP10) and 16% (CP16). Shade evaluations were conducted at baseline and then after 3 and 12 months of storage at 3 different temperatures: 10°C (±2°C), 25°C (±2°C), and 35°C (±2°C).

Restorations in primary teeth: a systematic review on survival and reasons for failures.

Background: Several restorative materials with specific indications are used for filling cavities in primary teeth.

Aim: To systematically review the literature in order to investigate the longevity of primary teeth restorations and the reasons for failure.

Design: Electronic databases were screened, and eligible studies were hand-searched to find longitudinal clinical studies evaluating the survival of restorations (class I, class II, and crown) placed with different materials in primary teeth with at least one year of follow-up.

Venous Blood Derivatives as FBS-Substitutes for Mesenchymal Stem Cells: A Systematic Scoping Review.

Although the biological properties of mesenchymal stem cells (MSC) are well-characterized in vitro, MSC clinical application is still far away to be achieved, mainly due to the need of xenogeneic substances for cell expansion, such as fetal bovine serum (FBS). FBS presents risks regarding pathogens transmissions and internalization of animal's proteins, which can unleash antigenic responses in patients after MSC implantation. A wide range of venous blood derivatives (VBD) has been reported as FBS substitutes showing promising results.

Platelet-Poor Plasma as a Supplement for Fibroblasts Cultured in Platelet-Rich Fibrin.

The aim of this study was to evaluate the proliferation and adhesion of mesenchymal cells (3T3/NIH) in Dulbecco's Modified Eagle Medium(DMEM) supplemented with Platelet-Poor Plasma (PPP) in aPlatelet-Rich Fibrin (PRF) scaffold. Human blood was obtained and processed in a centrifuge considering the equation G=1.12xRx(RPM/1000) to obtain PRF and PPP.

Does Cryopreservation Affect the Biological Properties of Stem Cells from Dental Tissues? A Systematic Review.

This systematic review evaluated if different cryopreservation protocols could affect biological properties (Cell survival rate (CSR), proliferation, differentiation, maintenance of stem cell markers) of stem cells obtained from dental tissues (DSC) post-thaw. An electronic search was carried out within PubMed and ISI Web Science by using specific keyword. Two independent reviewers read the titles and abstracts of all reports respecting predetermined inclusion/exclusion criteria.

Immunohistochemical Expression of TGF-β1 and Osteonectin in engineered and Ca(OH)2-repaired human pulp tissues.

The aim of the present study was to evaluate the expression of transforming growth factor-β1 (TGF-β1) and osteonectin (ON) in pulp-like tissues developed by tissue engineering and to compare it with the expression of these proteins in pulps treated with Ca(OH)2 therapy. Tooth slices were obtained from non-carious human third molars under sterile procedures. The residual periodontal and pulp soft tissues were removed.

Electrochemical Cathodic Polarization, a Simplified Method That Can Modified and Increase the Biological Activity of Titanium Surfaces: A Systematic Review.

Background: The cathodic polarization seems to be an electrochemical method capable of modifying and coat biomolecules on titanium surfaces, improving the surface activity and promoting better biological responses.

Objective: The aim of the systematic review is to assess the scientific literature to evaluate the cellular response produced by treatment of titanium surfaces by applying the cathodic polarization technique.

Data, Sources, And Selection: The literature search was performed in several databases including PubMed, Web of Science, Scopus, Science Direct, Scielo and EBSCO Host, until June 2016, with no limits used.

Vital Pulp Therapies in Clinical Practice: Findings from a Survey with Dentist in Southern Brazil.

Studies based on dentists' clinical practice possess vital relevance to understand factors leading the clinicians to choose by a specific technique over another. This study investigated which clinical conduct therapies are adopted by dentists in front of deep caries. Was evaluated how the place of work, post-graduate training and years since complete graduation influenced their decisions.

Preferences on vital and nonvital tooth bleaching: a survey among dentists from a city of southern Brazil.

This study evaluated the preferences of general dentists regarding vital and nonvital tooth bleaching therapies and investigated whether the time of clinical practice and post-graduate training influence these options. A cross-sectional study was conducted using a questionnaire with closed questions applied to dentists (n=276) of a mid-sized city of the southern Brazil (Pelotas, RS). Information was collected regarding sociodemographic variables, level of specialization and time since graduation.

Novel experimental cements for use on the dentin-pulp complex.

This aim of this study was to evaluate the physicochemical and biological properties of novel experimental cements (Hybrid, Paste and Resin) based on synergistic combinations of existing materials, including pH, diametral tensile strength (DTS) and cytotoxicity comparing them with mineral trioxide aggregate (MTA - Angelus®) and a glass ionomer cement (GIC) developed at our laboratory. For the physicochemical and biological tests, specimens with standard dimensions were produced. pH measurements were performed with digital pH meter at the following time intervals: 3, 24, 48 and 72 h.

Dental pulp tissue engineering.

Dental pulp is a highly specialized mesenchymal tissue that has a limited regeneration capacity due to anatomical arrangement and post-mitotic nature of odontoblastic cells. Entire pulp amputation followed by pulp space disinfection and filling with an artificial material cause loss of a significant amount of dentin leaving as life-lasting sequelae a non-vital and weakened tooth. However, regenerative endodontics is an emerging field of modern tissue engineering that has demonstrated promising results using stem cells associated with scaffolds and responsive molecules.

Comparison between DNA obtained from buccal cells of the upper and lower gutter area.

This study compared quantitatively and qualitatively the DNA extracted from buccal cells collected from the upper or lower gutter areas. Buccal cells were collected from the upper (n=15) and lower gutter (n=15) region from 15 volunteers using a special cytobrush (Gentra), totaling 2 collections from each individual. DNA was extracted from the samples according to the manufacturer's instructions.

Onium salt reduces the inhibitory polymerization effect from an organic solvent in a model dental adhesive resin.

This study evaluated the effect of organic solvent concentration on the polymerization kinetics for a model dental adhesive resin containing a ternary photoinitiator system. A monomer blend based on the bis-GMA, TEGDMA, and HEMA was used as a model dental adhesive resin, which was polymerized using a binary system [camphorquinone (CQ) and ethyl 4-dimethylamine benzoate (EDAB)] and a ternary system [CQ, EDAB, and diphenyliodonium hexafluorphosphate (DPIHFP)]. Additionally, these blends had 0, 10, 20, 30, and 40 wt % ethanol added.