Remote ischemic preconditioning and tacrolimus in the fetal small bowel transplant in mice.

Purpose:: To evaluate the effect of remote ischemic preconditioning (IPC-R) in the fetal small bowel transplantation model.

Methods:: Two groups were constituted: The Isogenic transplant (ISO, C57BL/6 mice, n=24) and the allogenic transplant (ALO, BALB/c mice, n=24). In each group, the animals were distributed with and without IPC-R.

The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation.

Purpose: To evaluate the NAC effects on liver hypothermic preservation at different time intervals.

Methods: For this, we used livers of male Wistar rats weighing between 250 and 300 g, undergoing preservation in Ringer solution at 4°C for up to 24 hours. Tissue samples were obtained at four moments of preservation for histological examination by hematoxylin and eosin staining: T0 = beginning of preservation, T12 = 12 hours, T18 = 18 hours and T24 = 24 hours.

Ischemic preconditioning attenuates remote pulmonary inflammatory infiltration of diabetic rats with an intestinal and hepatic ischemia-reperfusion injury.

Purpose: To assess ischemic preconditioning (IPC) effects in pulmonary lesion in intestinal and hepatic ischemia-reperfusion (IR) injury models using diabetic rats.

Methods: Diabetes (DM) was induced in 28 male Wistar rats by alloxan (42 mg/kg, IV). After 28 days, severe DM rats were submitted to intestinal or hepatic IR injury with or without IPC.

Effects of ischemic preconditioning associated to different preservation solutions in protecting the intestinal graft.

Purpose: To evaluate the effects of ischemic preconditioning (IPC) associate with different preservation solutions, in the protecting of gut.

Methods: Four groups of 14 rats underwent laparotomy and collecting 20 cm of ileum, for preservation, at 4ºC, in Belzer (Belz), Ringer (RL), Celsior (Cs) and Custodiol (Cust) solutions, for 24 hours. Prior to collection, half of the animals in each group were subjected to IPC.