Partitioning of porcine pancreatic lipase in a two-phase systems of polyethylene glycol/potassium phosphate aqueous.

The hydrolysis of triglycerides at the oil-water interface, synthesis of esters and transesterification in microaqueous conditions are catalysed by lipase. For its application, a proper purification method was necessary. This study examined the application of an aqueous two-phase system to partition porcine pancreatic lipase.