Multicolor fluorescence microscopy using static light sheets and a single-channel detection.

We present a multicolor fluorescence microscope system, under a selective plane illumination microscopy (SPIM) configuration, using three continuous wave-lasers and a single-channel-detection camera. The laser intensities are modulated with three time-delayed pulse trains that operate synchronously at one third of the camera frame rate, allowing a sequential excitation and an image acquisition of up to three different biomarkers. The feasibility of this imaging acquisition mode is demonstrated by acquiring single-plane multicolor images of living hyphae of Neurospora crassa.

Noise in adaptive interferometric fiber sensor based on population dynamic grating in erbium-doped fiber.

Experimental investigations of the main noise sources that limit the sensitivity of the adaptive interferometric all-fiber sensors operating in the communication wavelength region are reported. Adaptive properties (i.e.

Rapid spontaneous Raman light sheet microscopy using cw-lasers and tunable filters.

We perform rapid spontaneous Raman 2D imaging in light-sheet microscopy using continuous wave lasers and interferometric tunable filters. By angularly tuning the filter, the cut-on/off edge transitions are scanned along the excited Stokes wavelengths. This allows obtaining cumulative intensity profiles of the scanned vibrational bands, which are recorded on image stacks; resembling a spectral version of the knife-edge technique to measure intensity profiles.

Adaptive Sagnac interferometer with dynamic population grating in saturable rare-earth-doped fiber.

Sagnac fiber interferometer with the dynamic population grating formed in the rare-earth doped fiber is proposed for homodyne adaptive detection of optical phase modulation. The configuration is shown to be a simple all-optical fiber sensor suitable for linear high sensitivity detection of mechanical vibrations, acoustic signals, thermo-optic effect etc. Theoretical consideration shows that the quadratic response of this interferometric configuration associated with the amplitude dynamic grating is observed in the reflected wave mainly, while the recorded phase grating results in the linear energy exchange between the transmitted and reflected from the Sagnac loop light waves.