Machine Learning Based on Computed Tomography Pulmonary Angiography in Evaluating Pulmonary Artery Pressure in Patients with Pulmonary Hypertension.

Background: Right heart catheterization is the gold standard for evaluating hemodynamic parameters of pulmonary circulation, especially pulmonary artery pressure (PAP) for diagnosis of pulmonary hypertension (PH). However, the invasive and costly nature of RHC limits its widespread application in daily practice.

Purpose: To develop a fully automatic framework for PAP assessment via machine learning based on computed tomography pulmonary angiography (CTPA).

Comparative analysis of hydraulic refrigeration and mechanical vapour compression water cooling technologies in designing a technical system for oysters conservation.

The phenomenon of summer mortality in Pacific oysters cultures also occurs in Brazilian crops, with predominance in the adult phase, generating significant losses for local producers. In the search for a technological solution to mitigate its effects, the mechanical vapour compression and hydraulic refrigeration concepts are evaluated as two proposed cooling technologies. The comparative analysis carried out with numeric simulations indicated that the hydraulic cooling system presents disadvantages regarding both the size of the compression column and the energy efficiency, compared to the mechanical vapour compression cycle.

In vivo analysis of the acidic ribosomal proteins BmP1 and BmP2 of the silkworm Bombyx mori in the yeast Saccharomyces cerevisiae.

In the silkworm Bombyx mori the ribosomal stalk P-protein family consists of two low MW acidic proteins, BmP1 and BmP2, and of one higher MW protein, BmP0, as shown by electrophoretical and immunoblotting western blot analysis of purified ribosomes. Treatment of ribosomes with alkaline phosphatase followed by electrofocusing shifted the isoelectric points to higher pH, implying phosphorylation of the proteins. The cDNAs encoding BmP1 and BmP2 proteins were constructed and expressed in the Saccharomyces cerevisiae mutant strains defective in either the endogenous P1 or P2 proteins.

Different roles of P1 and P2 Saccharomyces cerevisiae ribosomal stalk proteins revealed by cross-linking.

The stalk is an essential domain of the large ribosomal subunit formed by a complex of a set of very acidic proteins bound to a core rRNA binding component. While in prokaryotes there is only one type acidic protein, L7/12, two protein families are found in eukaryotes, phosphoproteins P1 and P2, which presumably have different roles. To search for differences zero-length cross-linking by S-S bridge formation was applied using Saccharomyces cerevisiae mutant P1 and P2 proteins carrying single cysteine residues at various positions.