Automated workflow utilizing saponification and improved epoxidation for the sensitive determination of mineral oil saturated and aromatic hydrocarbons in edible oils and fats.

Refined edible oils and fats are known to contain olefins resisting the typical epoxidation used for the sample preparation of mineral oil saturated and aromatic hydrocarbons (MOSH and MOAH). These olefins can be misinterpreted as MOAH and are therefore an important reason for inconsistent results between laboratories. Collaborative trials confirm this assumption for low MOAH contents near the quantitation limits regularly.

Determination of mineral oil aromatic hydrocarbons in edible oils and fats by online liquid chromatography-gas chromatography-flame ionization detection - Evaluation of automated removal strategies for biogenic olefins.

The determination of mineral oil aromatic hydrocarbons (MOAH) in foodstuffs gained in importance over the last years as carcinogenicity cannot be excluded for certain MOAH. The existence of olefins in foodstuffs, such as edible oils and fats, can be problematic for the determination of MOAH by LC-GC-FID. Removal of these interfering substances by HPLC based on polarity differences is not possible.

Fully automated determination of the sterol composition and total content in edible oils and fats by online liquid chromatography-gas chromatography-flame ionization detection.

Sterol analysis of edible oils and fats is important in authenticity control. The gas chromatographic determination of the sterol distribution and total content is described by ISO norm 12228. Extraction, purification, and detection of the sterols are time-consuming and error-prone.

Universal Route to Polycyclic Aromatic Hydrocarbon Analysis in Foodstuff: Two-Dimensional Heart-Cut Liquid Chromatography-Gas Chromatography-Mass Spectrometry.

Analysis of polycyclic aromatic hydrocarbons (PAHs) in complex foodstuff is associated with complicated and work-intensive sample preparation. Chromatographic interference has to be faced in many situations. The scope of the current work was the development of a highly efficient two-dimensional heart-cut LC-LC-GC-MS method.

Determination of vitamins D2 and D3 in selected food matrices by online high-performance liquid chromatography-gas chromatography-mass spectrometry (HPLC-GC-MS).

An online normal-phase liquid chromatography-gas chromatography-mass spectrometry (HPLC-GC-MS) method was developed for the determination of vitamins D2 and D3 in selected food matrices. Transfer of the sample from HPLC to GC was realized by large volume on-column injection; detection was performed with a time-of-flight mass spectrometer (TOF-MS). Typical GC problems in the determination of vitamin D such as sample degradation or sensitivity issues, previously reported in the literature, were not observed.

Determination of polychlorinated biphenyls and organochlorine pesticides in small volumes of human blood by high-throughput on-line SPE-LVI-GC-HRMS.

A fully automated and robust method featuring on-line solid-phase extraction (SPE) and large volume injection (LVI) gas chromatographic (GC) high resolution mass spectrometry (HRMS) is used to determine polychlorinated biphenyls (PCBs) and organochlorine pesticides, such as penta- and hexachlorobenzene (PeCBz, HxCBz), hexachlorocyclohexane isomers (HCH) and 4,4'-dichlorodiphenyldichloroethene (a metabolite of dichlorodiphenyltrichloroethane (DDT)), with only 200μl of human blood, serum or plasma. After spiking the sample with (13)C-labeled internal standards and precipitating the proteins, the sample is passed through a 10mm×2.0mm ID SPE cartridge filled with C18 material that adsorbs the analytes.