Publications by authors named "Marco C Rotondi"
Article Synopsis
- - The study analyzed human mesenchymal stromal cells (hMSCs) from adipose tissue, bone marrow, and umbilical cord to compare their growth and functionality across multiple passages.
- - Results showed that umbilical cord-derived hMSCs (UCT-hMSCs) proliferated faster than adult sources (adipose and bone marrow), and bone marrow-hMSCs produced more vascular endothelial growth factor (VEGF).
- - Despite differences in growth and functionality, all hMSC sources expressed similar markers for identification, suggesting a systematic method for selecting the best cell source based on clinical needs.
Due to their immunomodulatory properties and in vitro differentiation ability, human mesenchymal stromal cells (hMSCs) have been investigated in more than 1000 clinical trials over the last decade. Multiple studies that have explored the development of gene-modified hMSC-based products are now reaching early stages of clinical trial programmes. From an engineering perspective, the challenge lies in developing manufacturing methods capable of producing sufficient doses of ex vivo gene-modified hMSCs for clinical applications.
View Article and Find Full Text PDFChimeric antigen receptor T-cell (CAR-T) therapies have proven clinical efficacy for the treatment of hematological malignancies. However, CAR-T cell therapies are prohibitively expensive to manufacture. The authors demonstrate the manufacture of human CAR-T cells from multiple donors in an automated stirred-tank bioreactor.
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