Publications by authors named "Marco Bellotti"
Oral immunization offers a minimally invasive administration, inducing local and systemic immune responses and facilitating mass immunization without needle-related risks. However, the gastrointestinal environment poses challenges, compromising vaccine effectiveness through enzymatic degradation and poor absorption by Peyer's patches. Advances in nanoparticle and microparticle (NP/MP) technology protect vaccines from degradation and enhance targeted release.
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- The microfluidic production of nanoparticles (NPs) is gaining popularity as a more efficient and controllable alternative to traditional methods like nanoprecipitation and emulsification.
- Developing new formulations for NPs requires extensive and costly experimentation, but a computational fluid dynamic pipeline was created to streamline this process.
- The study validated this computational approach by correlating numerical and experimental results, demonstrating its potential to optimize the NP manufacturing process, particularly in managing size heterogeneity and mixing efficiency.
Lipid-based nanocarriers have emerged as helpful tools to deliver sensible biomolecules such as proteins and oligonucleotides. To have a fast and robust microfluidic-based nanoparticle synthesis method, the setup of versatile equipment should allow for the rapid transfer to scale cost-effectively while ensuring tunable, precise and reproducible nanoparticle attributes. The present work aims to assess the effect of different micromixer geometries on the manufacturing of lipid nanocarriers taking into account the influence on the mixing efficiency by changing the fluid-fluid interface and indeed the mass transfer.
View Article and Find Full Text PDFNanomedicine consists in the application of nanotechnology in medicine to revolutionize the healthcare sector through transformative new diagnostic and therapeutic tools. In this field, nanostructures or nanocarriers (i.e.
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- The study aimed to compare quantitative PCR (qPCR) with two different digital PCR (dPCR) systems in measuring three bacterial pathogens.
- Results showed that both dPCR methods provided consistent quantification, while qPCR had varying degrees of overestimation or underestimation compared to dPCR.
- For one specific pathogen, both cultural methods and PCR approaches yielded similar quantification, indicating that PCR can be a reliable alternative to traditional methods for that pathogen.