Recent advances in recombinant production of soluble proteins in E. coli.

Background: E. coli still remains the most commonly used organism to produce recombinant proteins in research labs. This condition is mirrored by the attention that researchers dedicate to understanding the biology behind protein expression, which is then exploited to improve the effectiveness of the technology.

Dynamic PRC1-CBX8 stabilizes a porous structure of chromatin condensates.

The compaction of chromatin is a prevalent paradigm in gene repression. Chromatin compaction is commonly thought to repress transcription by restricting chromatin accessibility. However, the spatial organization and dynamics of chromatin compacted by gene-repressing factors are unknown.

Thoracoabdominal chordoma in a pediatric patient. A rare entity.

Introduction: Chordoma is a rare, slow-growing notochordal neoplasm typical of adults. Less than 5% of the cases occur in children, where they are located at the skull base. Treatment involves surgical resection with or without radiotherapy.

Nocardiosis in domestic ferrets (Mustela putorius furo).

Nocardia spp are ubiquitous, gram-positive, variably acid-fast, branching and beaded filamentous, facultative intracellular bacteria that are resistant to phagocytosis and can cause localized or systemic disease in a variety of mammals, including humans, as well as in birds, fish and reptiles. Seventeen pet domestic ferrets (Mustela putorius furo) were diagnosed with nocardiosis by several methods including cytological evaluation, histopathology, Ziehl-Neelsen staining and polymerase chain reaction (PCR). All except two ferrets were 2 years old or older at the time of clinical presentation.

Design and Test of a Calibration System for Avalanche Photodiodes Used in X-Ray Compton Polarimeters for Space.

The development and calibration of a measurement system designed for assessing the performance of the avalanche photodiodes (APDs) used in the Compton scattering polarimeter of the CUSP project is discussed in this work. The designed system is able to characterize the APD gain GAPD and energy resolution across a wide range of temperatures (from -20 °C to +60 °C) and bias voltages Vbias (from 260 V to 410 V). The primary goal was to experimentally determine the GAPD dependence on the and Vbias in order to establish a strategy for stabilizing GAPD by compensating for fluctuations, acting on Vbias.

Deep Septal Pacing for Pacemaker-Induced Cardiomyopathy.

Introduction: Right ventricular (RV) pacing can impair left ventricular function and cause heart failure, known as pacing-induced cardiomyopathy (PICM). Upgrade to cardiac resynchronization (CRT) is its usual treatment; recently left bundle branch area pacing (LBBAP) has emerged as a potential alternative. Deep septal pacing (DSP), a simplified alternative to LBBAP, is still able to achieve narrower paced QRS than during conventional RV pacing.

The nucleocapsid architecture and structural atlas of the prototype baculovirus define the hallmarks of a new viral realm.

Baculovirus is the most studied insect virus owing to a broad ecological distribution and ease of engineering for biotechnological applications. However, its structure and evolutionary place in the virosphere remain enigmatic. Using cryo-electron microscopy, we show that the nucleocapsid forms a covalently cross-linked helical tube protecting a highly compacted 134-kilobase pair DNA genome.

A Novel Substituted Benzo[]quinoxaline-Based Cyclometalated Ru(II) Complex as a Biocompatible Membrane-Targeted PDT Colon Cancer Stem Cell Agent.

Herein, we describe and investigate biological activity of three octahedral ruthenium(II) complexes of the type [Ru(CN)(phen)], -, containing a π-expansive cyclometalating substituted benzo[]quinoxaline ligand (CN ligand) (phen = 1,10-phenanthroline). Compounds - in cervical, melanoma, and colon human cancer cells exhibit high phototoxicity after irradiation with light (particularly blue), with the phototoxicity index reaching 100 for the complex in most sensitive HCT116 cells. accumulates in the cellular membranes.

Amplified Light-Induced p Modulation with Diarylethene Photoswitches.

The reversible modulation of acidity using molecular photoswitches enables the remote control of a variety of (bio)chemical processes with light. Herein we investigated the structural features that allow amplifying photoinduced p variation in phenol-diarylethene conjugates, which toggle between low- and high-acidity states by switching the conjugation between the ionizable moiety and electron-withdrawing groups upon photoisomerization. By tuning the structure of these conjugates, high p modulation amplitudes were accomplished that surpass those previously reported.

Inconsistency between process-based model and dose-response function in estimating biomass losses in Northern Hemisphere due to elevated O.

Tropospheric ozone (O) concentrations in the Northern Hemisphere have significantly increased since the pre-industrial era, with ongoing growth driven by emissions from industrial, agricultural, and transportation activities, further exacerbated by the warming temperatures and altered atmospheric circulation patterns associated with climate change. This study compared different methodologies for estimating biomass potential losses (BPL) in forests due to elevated O using both concentration-based (AOT40) and flux-based (POD1) metrics. Moreover, to further assess the impact of O on forest health and carbon uptake across the dominant forest types in the Northern Hemisphere, we also compared BPL estimates from dose-response functions with those derived from the process-based model ORCHIDEE.

Computational study of the HLTF ATPase remodeling domain suggests its activity on dsDNA and implications in damage tolerance.

The Helicase-Like Transcription Factor (HLTF) is member of the SWI/SNF-family of ATP dependent chromatin remodellers known primarily for maintaining genome stability. Biochemical and cellular assays support its multiple roles in DNA Damage Tolerance. However, the lack of sufficient structural data limits the comprehension of the molecular basis of its modes of action.

Physiological plasticity and life history traits affect Chamelea gallina acclimatory responses during a marine heatwave.

The striped venus clam (Chamelea gallina) is a relevant economic resource in the Adriatic Sea. This study explored the physiological status of C. gallina at four sites selected along a gradient from high to low incidence of recorded historical mortality events and low to high productivity in the Northwestern Adriatic Sea.

Skin metastasis of BRCA mutated prostate cancer: A case report and a brief review of literature.

Rationale: Metastatic castration-resistant prostate cancer has a poor prognosis especially when harboring DNA damage repair gene mutations, nevertheless, in the case of pathogenic BRCA gene mutations, PARPi demonstrated a survival benefit and is a validated treatment. Nowadays, there is no data regarding unusual metastases after these drugs. Cutaneous metastases appear rarely in prostate cancer and were associated with a worse prognosis.

A smartphone app for preschool wheezing and reliability of medical history collection.

Background: The use of mobile applications helps improving self-management in adolescents with asthma. However, no evidence is available for children with preschool wheezing. In addition, we have no data on the reliability of medical history collected at visits.

Corrosion on cultural heritage buildings in Jordan in current situation and in future climate scenarios.

This study examines the impact of air pollution on Jordan's cultural heritage sites, focusing on key pollutants (SO, HNO, O, PM10) and climate conditions. Using 2019 data and future projections for 2040-2059 and 2080-2099, the research reveals significant material corrosion in urban areas like Amman and Irbid, driven by pollutants such as SO₂ and PM10. Random Forest Analysis identifies these pollutants as primary contributors to material degradation.

VP3.15 reduces acute cerebellum damage after germinal matrix-intraventricular hemorrhage of the preterm newborn.

Germinal matrix-intraventricular hemorrhage (GM-IVH) is one of the most common complications of the preterm newborn. The pathology of the GM-IVH is not completely understood and even regions distant from the lesion area are severely affected. It has been suggested that cerebellar diaschisis may underlie the neurodevelopmental problems that many of these kids show, including cerebral palsy, attention deficit disorders or hyperactivity.