Withanolide derivatives: natural compounds with anticancer potential offer low toxicity to fertility and ovarian follicles in mice.

Anticancer therapy often leads to premature ovarian insufficiency (POI) and infertility due to the extreme sensitivity of the ovarian follicle reserve to the effects of chemotherapy. Withanolides are known for their cytotoxic effect on cancer cells and low cytotoxicity on non-malignant or healthy cells. Therefore, this study aimed to investigate the effects of three withanolides derivatives: 27-dehydroxy-24,25-epoxywithaferin A (WT1), 27-dehydroxywithaferin A (WT2), and withaferin A (WTA) on fertility, and the ovarian preantral follicles of young female mice.

Systematic review and meta-analysis on patented and non-patented vitrification processes to ovarian tissue reported between 2000 and 2021.

Due to the great interest in ovarian cryopreservation and, consequently conservation and restoration of female fertility in the last decades, different vitrification procedures (vitrification devices or solutions) have been developed, patented, and used both for academic research purposes and for clinical use. Therefore, the present study aimed to provide a systematic review and meta-analysis of data obtained from the application of different patented and non-patented vitrification devices and solutions in different countries. For this purpose, relevant observational studies published between the years 2000 to 2021 were selected to verify the efficiency of ovarian vitrification processes on parameters such as morphology, viability, and apoptosis in preantral ovarian follicles after transplantation or in vitro culture.

Zinc oxide nanoparticles functionalized with curcumin supplementation during in vitro embryo culture impaired in a concentration-dependent-manner blastocyst production in cattle.

This work investigated the effect of zinc oxide nanoparticles functionalized with curcumin (ZnO  + CUR) supplementation during the in vitro embryo culture (IVC) on the bovine in vitro embryo production, and the cellular antioxidant response. The cumulus-oocyte complexes (COCs) were matured, fertilized and then the presumptive zygotes were cultured in the medium in the absence (0 μM-control) or presence of different concentrations of ZnO  + CUR (3, 6 or 12 μM). After IVC, the embryos were destined either to assay intracellular ROS levels and mitochondrial membrane potential.

Analysis of false positive PI-RADS 4 lesions: experience from a single nonacademic center using cognitive fusion.

Background: We evaluated pathological findings in targeted biopsies of PI-RADS4 and PI-RADS5 lesions, and clinical data that could predict those patients with benign findings.

Materials And Methods: A retrospective study was conducted to summarize the experience from a single nonacademic center using cognitive fusion and a 1.5 or 3.

Seminal quality comparison of first and second cryopreserved ejaculates of Alpine kid goats by flow cytometry.

Discarding the first ejaculate is recommended as an alternative for improving seminal quality after long sexual resting, especially when semen should be used for cryopreservation. However, when the males are not in sexual resting the necessity to discarding the first ejaculate is still unknown. Therefore, this study aimed to compare by flow cytometry the quality of the first and second ejaculates.

Real life data of MRI-targeted biopsy - experience from a single nonacademic centre using cognitive fusion and 1.5 tesla scanning.

Objectives: To date, it is unknown whether systematic biopsies can be safely omitted in patients with unsuspicious MRI findings or if systematic biopsies should be required when targeting focal lesions (PI-RADS 3-5).

Methods: A series of 366 patients (249 without a previous biopsy) were examined in a 1.5 Tesla MRI scanner.

Use of Castor Bean Meal, Biodiesel Industry Coproduct, in A Lamb Production System Using Creep-Feeding in Brazil.

This study aimed to evaluate the replacement of soybean meal by detoxified castor bean meal on the intake and digestibility of nutrients, body weight gain, carcass yield, physiological and urinary parameters, and creep-feeding method effectiveness. For this trial, 43 male lambs were used, Dorper × Santa Inês, with an average initial body weight of 7.95 kg, 15 days old.

Can maintenance intake reduce production costs without compromising the reproductive performance in bucks?

Excessive intake of nutrients can result in decreases in the reproductive efficiency of goat bucks and increase production costs in goat e nterprises. In this study, the aim was to compare the reproductive features of young bucks fed diets for maintenance of growth or were fed ad libitum. Ten bucks were used (n = 5/group), with a completely randomized design.

Effect of phase-feeding crude protein on performance and carcass characteristics of crossbred beef bulls: an application to reduce nitrogen compounds in beef cattle diets.

Beef cattle (24) with an average initial body weight of 417 ± 54 kg were assigned in a 2 × 2 factorial design with six repetitions. The factors studied were two levels of CP: 11 (low CP) or 13 % (high CP) in the initial feedlot phase (days 1 - 36) and in the final phase (days 37 - 72). After 36 days, half of the bulls fed with each level of CP were selected to reverse the CP level.