Cytotoxicity, antibacterial and physicochemical properties of a new epoxy resin-based endodontic sealer containing calcium hydroxide.

Background: This study evaluated the cytotoxicity, antibacterial and physicochemical properties of a new epoxy resin-based endodontic sealer containing calcium-hydroxide Sealer Plus. AH Plus was used as a reference for comparison.

Material And Methods: Cytotoxicity evaluation was performed according to ISO-10993-5 specifications using MTT assay to check the 3T3 cells viability at 1- to 4-week periods.

Evaluation of Influence of Widening Apical Preparation of Root Canals on Efficiency of Ethylenediaminetetraacetic Acid Agitation Protocols: Study by Scanning Electron Microscopy.

Aim: The purpose of this study was to evaluate the influence of widening the apical root canal preparation (RCP) on the efficiency of different ethylenediaminetetraacetic acid (EDTA) agitation protocols on smear layer removal: EDTA; EDTA + Easy Clean in rotary movement (ECROT); EDTA + Easy Clean in reciprocating movement (ECREC); and EDTA + Passive ultrasonic irrigation (PUI).

Materials And Methods: A total of 80 mandibular premolars had their crowns sectioned and then were divided into two groups according to widening: size 25 or 40, 0.08 taper.

Effectiveness of the ProTaper Next and Reciproc Systems in Removing Root Canal Filling Material with Sonic or Ultrasonic Irrigation: A Micro-computed Tomographic Study.

Introduction: The aim of this study was to evaluate the effectiveness of ProTaper Next (Dentsply Maillefer, Ballaigues, Switzerland) and Reciproc (VDW, Munich, Germany) systems in removing filling material from oval root canals using sonic or ultrasonic irrigation as additional cleaning methods.

Methods: Thirty-two human extracted mandibular premolars with oval canals were prepared using the ProTaper Universal system (Dentsply Maillefer) up to instrument F4 (40/.06) and then filled by the single-cone technique using Endofill sealer (Dentsply Maillefer).

Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment.

Objective: The antimicrobial effect of ultrasonic agitation of calcium hydroxide (CH) pastes in infected bovine dentin and their penetrability were evaluated using confocal laser scanning microscopy (CLSM) and microbiological culture.

Material And Methods: Fifty-two bovine teeth were infected with Enterococcus faecalis using a new contamination protocol; then they received CH paste and were divided into groups with or without ultrasound. Ultrasonic agitation was conducted for 1 min with a plain point insert.

Effect of the time-point of acid etching on the persistence of sealer residues after using different dental cleaning protocols.

Endodontic sealer residues remaining within the pulp chamber dentin after root canal obturation and cleaning with various solvents may compromise the appearance and the durability of dental restorations. Acid etching is routinely performed prior to application of dentine adhesive systems, but is effect on residual sealer material and the optimal time-point for performing etching, are unknown. Here, we evaluated the effect of acid etching on the dentin surface when performed either immediately or 7 days after removal of the endodontic sealer with two solvents, i.

Comparative Effectiveness of New Mechanical Irrigant Agitating Devices for Debris Removal from the Canal and Isthmus of Mesial Roots of Mandibular Molars.

Introduction: The aim of this study was to compare the effectiveness of Easy Clean (Easy Dental Equipment, Belo Horizonte, MG, Brazil) in continuous and reciprocating motion, passive ultrasonic irrigation (PUI), Endoactivator systems (Dentsply Maillefer, Ballaigues, Switzerland), and conventional irrigation for debris removal from root canals and isthmus.

Methods: Fifty mesial roots of mandibular molars were embedded in epoxy resin using a metal muffle; afterward, the blocks containing the roots were sectioned at 2, 4, and 6 mm from the apex. After instrumentation, the roots were divided into 5 groups (n = 10) for application of the final irrigation protocol using Easy Clean in continuous rotation, Easy Clean in reciprocating motion, PUI, Endoactivator, and conventional irrigation.

Evaluation of Different Passive Ultrasonic Irrigation Protocols on the Removal of Dentinal Debris from Artificial Grooves.

The aim of this study was to evaluate the influence of different passive ultrasonic irrigation protocols on the removal of debris in artificially created grooves in the cervical, middle and apical root thirds. Forty extracted bovine incisor roots were instrumented to 1 mm of the root apex with a R50 Reciproc instrument and irrigated with 2.5% sodium hypochlorite.

Effect of the Association of Nonsteroidal Anti-inflammatory and Antibiotic Drugs on Antibiofilm Activity and pH of Calcium Hydroxide Pastes.

Introduction: The objective of the present study was to evaluate the in vitro antibiofilm activity and pH of calcium hydroxide associated with different nonsteroidal anti-inflammatory drugs (NSAIDs).

Methods: The groups analyzed were as follows: group 1, calcium hydroxide paste with propylene glycol; group 2, calcium hydroxide paste with propylene glycol + 5% diclofenac sodium; group 3, calcium hydroxide paste with propylene glycol + 5% ibuprofen; group 4, calcium hydroxide paste with propylene glycol + 5% ciprofloxacin; and group 6, positive control (without medication). For analysis of the pH, the pastes were inserted into tubes and immersed in flasks containing ultrapure water.

Intratubular disinfection with tri-antibiotic and calcium hydroxide pastes.

Objective: The aim of this study was to compare the in vitro intradentinal antimicrobial ability of the calcium hydroxide and tri-antibiotic pastes.

Materials And Methods: Standard bovine dentin tubes were sterilized and then infected with Enterococcus faecalis by a new contamination protocol of great depths of dentin. The specimens were filled with the medications, divided into two test-groups: calcium hydroxide (Group 1) and tri-antibiotic (Group 2) pastes.

Prevalence and morphometric analysis of three-rooted mandibular first molars in a Brazilian subpopulation.

Objectives:: To determine the prevalence of three-rooted mandibular molars in a Brazilian population using cone beam computed tomography (CBCT) and to analyze the anatomy of mandibular first molars with three roots through micro-CT.

Material And Methods:: CBCT images of 116 patients were reviewed to determine the prevalence of three-rooted first mandibular molars in a Brazilian subpopulation. Furthermore, with the use of micro-CT, 55 extracted three-rooted mandibular first molars were scanned and reconstructed to assess root length, distance between canal orifices, apical diameter, Vertucci's classification, presence of apical delta, number of foramina and furcations, lateral and accessory canals.

Efficacy of CM-Wire, M-Wire, and Nickel-Titanium Instruments for Removing Filling Material from Curved Root Canals: A Micro-Computed Tomography Study.

Introduction: The aim of this ex vivo study was to evaluate the removal of filling material after using CM-wire, M-wire, and nickel-titanium instruments in both reciprocating and rotary motions in curved canals.

Methods: Thirty maxillary lateral incisors were divided into 9 groups according to retreatment procedures: Reciproc R25 followed by Mtwo 40/.04 and ProDesign Logic 50/.

Changes in Root Canal Length Determined during Mechanical Preparation Stages and Their Relationship with the Accuracy of Root ZX II.

Introduction: The aim of this study was to determine the variations in root canal length (RCL) occurring during endodontic treatment stages (initial, preflared, and concluded) and correlate them with the accuracy of Root ZX II (RZX).

Methods: After coronal access, 26 mandibular molars had the apical foramen of the 52 mesial canals standardized (250 μm) and their respective initial RCL was recorded (RCL1 = initial) by using a clinical microscope (×16) and manual K-file instruments. By using the alginate model, sequential electronic measurements were taken with the RZX.

Effect of time and ultrasonic activation on ethylenediaminetetraacetic acid on smear layer removal of the root canal.

The aim of this study was to assess the effect of time and the ultrasonic activation of ethylenediaminetetraacetic acid (EDTA) in removing the smear layer of at root canal. A total of 60 bovine inferior incisors were transversely sectioned and instrumented; A smear layer was added in the root dentin surface. The specimen were then divided into six groups according to the following final irrigation protocols: Group (G) 1: conventional irrigation with sodium hypochlorite (NaOCl); G2: conventional irrigation with NaOCl + EDTA for 20 s; G3: conventional irrigation with NaOCl + EDTA for 60 s; G4: passive ultrasonic irrigation (PUI) with NaOCl; G5: PUI with NaOCl + PUI with EDTA for 20 s; G6: PUI + NaOCl and PUI with EDTA for 60 s.

Antimicrobial Activity and Physicochemical Properties of Calcium Hydroxide Pastes Used as Intracanal Medication.

Introduction: The aim of the present study was to evaluate the pH, calcium release, solubility, and antimicrobial action against biofilms of calcium hydroxide + saline solution, Calen (SS White Artigos Dentários Ltd, Rio de Janeiro, Brazil) (CH/P), Calen camphorated paramonochlorophenol (CMCP) (CH/CMPC), and calcium hydroxide + chlorhexidine (CH/CHX) pastes.

Methods: The pH of the pastes was determined with a calibrated pH meter placed in direct contact with each paste. The root canals of acrylic teeth (N = 10) were filled with the previously mentioned intracanal dressings and immersed in ultrapure water to measure hydroxyl (pH meter) and calcium ion release (atomic absorption spectrophotometer) at time intervals of 3, 7, 15, and 30 days.

Tissue dissolution and modifications in dentin composition by different sodium hypochlorite concentrations.

Objectives: To determine the organic matter dissolution and changes in dentin chemical composition promoted by different concentrations of NaOCl over time.

Material And Methods: Fragments of bovine muscle tissue were weighed before and after 5, 10, and 15 min of immersion in the groups (n=10): G1- 0.9% saline solution; G2- 1% NaOCl; G3- 2.

Push-out Bond Strength of Root-end Filling Materials.

The aim of this study was to evaluate the bond strength of root-end filling materials. Forty 2-mm-thick slices were obtained from human single-rooted teeth. After root canal preparation using a 1.

Ions Release and pH of Calcium Hydroxide-, Chlorhexidine- and Bioactive Glass-Based Endodontic Medicaments.

This study evaluated pH and release of calcium, sodium and phosphate ions from different medications in human dentin. Fifty premolars were prepared and randomly divided into groups: (CHX) - 2% chlorhexidine gel; (CHX + CH) - CHX + calcium hydroxide PA; (CH) - CH + propylene glycol 600; (NPBG) - experimental niobium phosphate bioactive glass + distilled water; (BG) - bioactive glass (Bio-Gran) + distilled water. The specimens were immersed in deionized water and the pH variations were measured.

Sealing ability of MTA, CPM, and MBPc as root-end filling materials: a bacterial leakage study.

Objectives To evaluate the sealing ability of three root-end filling materials (white MTA, CPM, and MBPc) using an Enterococcus faecalis leakage model. Material and Methods Seventy single-root extracted human teeth were instrumented and root-ends were resected to prepare 3 mm depth cavities. Root-end preparations were filled with white MTA, CPM, and MBPc cements.

Micro-CT Evaluation of Root Filling Removal after Three Stages of Retreatment Procedure.

The aim of this study was to quantify the residual filling material after filling removal, re-preparation with rotary or reciprocating files and passive ultrasonic irrigation (PUI). Twenty maxillary molars were prepared using ProTaper instruments up to F1. The teeth were filled with AH Plus and ProTaper gutta-percha points using the single-cone technique.

Experimental Calcium Silicate-Based Cement with and without Zirconium Oxide Modulates Fibroblasts Viability.

The aim of this study was to verify whether the use of zirconium oxide as a radiopacifier of an experimental calcium silicate-based cement (WPCZO) leads to cytotoxicity. Fibroblasts were treated with different concentrations (10 mg/mL, 1 mg/mL, and 0.1 mg/mL) of the cements diluted in Dulbecco's modified Eagle's medium (DMEM) for periods of 12, 24, and 48 h.

The effects of chlorhexidine and ethanol on push-out bond strength of fiber posts.

Context: Irrigation of root canals with chlorhexidine (CHX) and ethanol is common practice to prevent root canal infection during postplacement. However, pretreatment with these solvents may interfere with the bond strength of posts.

Aims: This study aimed to evaluate if root dentin pretreatment using CHX and/or ethanol influences the push-out bond strength of fiber-reinforced composite resin (FRCR) posts.

Calcium silicate-based sealers: Assessment of physicochemical properties, porosity and hydration.

Objectives: Investigation of hydration, chemical, physical properties and porosity of experimental calcium silicate-based sealers.

Methods: Experimental calcium silicate-based sealers with calcium tungstate and zirconium oxide radio-opacifiers were prepared by mixing 1g of powder to 0.3 mL of 80% distilled water and 20% propylene glycol.

Efficacy of Electronic Foramen Locators in Controlling Root Canal Working Length during Rotary Instrumentation.

The present study evaluated the efficacy of electronic foramen locators (EFLs) to control root canal working length during rotary instrumentation and to assess possible reliability variations of different working lengths. Forty-eight human mandibular bicuspids were randomly divided in 2 groups according to the used device, Root ZX II (RZX) and Propex II (PRO). They were further subdivided in 2 subgroups according to the root canal preparation level (0.