Publications by authors named "Marcio R Cepeda"
Article Synopsis
- An instant coffee extract rich in arabinogalactans was shown to stimulate B lymphocytes in mice through in vitro studies.
- The active compounds primarily elute near the void volume in size-exclusion chromatography and remain effective even after chymotrypsin treatment.
- However, the removal of terminal arabinose residues decreased B cell activation, indicating their importance in the immunostimulatory effects related to coffee.
Instant coffee was prepared and fractionated into higher (>100kDa), medium (5-10, 10-30, 30-100kDa) and lower (1-5, <1kDa) molecular weight fractions. Sugars and linkage composition characteristics of arabinogalactans and galactomannans were recovered in all fractions. Also, amino acid analysis performed after hydrolysis showed similar compositions in all fractions.
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