DNA-based molecular recognition system for lactoferrin biosensing.

The paper describes the development of a novel DNA oligonucleotide-based affinity bioreceptor that binds to lactoferrin, a glycoprotein-type immunomodulator. The research was performed using surface plasmon resonance method to investigate affinity of various types of oligonucleotides to the target protein. The 72 base pair-long 5'[(TAGAGGATCAAA)AAA]TAGAGGATCAAA3' sequence with the highest affinity to lactoferrin was selected for further investigations.

Effect of dendrimer-based interlayers for enzyme immobilization on a model electrochemical sensing system for glutamate.

In this paper, we discuss dendrimer usage in enzyme-based electrochemical biosensors, particularly with respect to biomolecule loading on the sensing surface. A novel approach to design bioactive layers with immobilized enzymes for electrochemical biosensors using the surface plasmon resonance (SPR) method in combination with electrochemical impedance spectroscopy was presented. The gold surface was modified with linear linkers (various mercaptoalkanoic acids and aminoalkanethiols) and poly(amidoamine) dendrimers from the first- to fifth-generation.

Biosensor based on coupled enzyme reactions for determination of arginase activity.

The determination of the enzymatic activity requires constant and reproducible measuring conditions, therefore highly stable potentiometric biosensor operating on the basis of coupled enzyme reactions is proposed for arginase activity determination. Glassy carbon electrode was covered with polyazulene ion-to-electron transducing layer, which ensured improved stability of the prepared sensor. The sensor's selectivity was obtained by applying NH-selective membrane on the transducing layer, which was further biofunctionalized with urease via covalent immobilization.

Highly Stable Potentiometric (Bio)Sensor for Urea and Urease Activity Determination.

There is growing interest for bioanalytical tools that might be designed for a specific user, primarily for research purposes. In this perspective, a new, highly stable potentiometric sensor based on glassy carbon/polyazulene/NH-selective membrane was developed and utilized for urease activity determination. Urease-urea interaction studies were carried out and the Michaelis-Menten constant was established for this enzymatic reaction.

Potentiometric Solid-Contact Ion-Selective Electrode for Determination of Thiocyanate in Human Saliva.

A new solid-contact potentiometric ion-selective electrode for the determination of SCN (SCN-ISE) has been described. Synthesized phosphonium derivative of calix[4]arene was used as a charged ionophore. The research included selection of the ion-selective membrane composition, determination of the ISEs metrological parameters and SCN-ISE application for thiocyanate determination in human saliva.