Characterization of First Races in Sri Lanka and Identification of Genes for the Development of Rust-Resistant Snap Beans.

Bean rust is a devastating disease of snap beans in Sri Lanka. Our study endeavored to develop snap beans with resistance to , the bean rust pathogen reported to have numerous virulent races. Accordingly, we needed a detailed understanding of the virulence of .

Different loci control resistance to different isolates of the same race of Colletotrichum lindemuthianum in common bean.

Linkage and genome-wide association analyses using high-throughput SNP genotyping revealed different loci controlling resistance to different isolates of race 65 of Colletotrichum lindemuthianum in common bean. Development of varieties with durable resistance to anthracnose is a major challenge in common bean breeding programs because of the extensive virulence diversity of Colletotrichum lindemuthianum fungus. We used linkage and genome-wide association analyses to tap the genomic regions associated with resistance to different isolates of race 65.

Fine mapping of an anthracnose-resistance locus in Andean common bean cultivar Amendoim Cavalo.

Anthracnose, caused by the fungal pathogen Colletotrichum lindemuthianum, is one of the world's most destructive diseases of common bean. The use of resistant cultivars is the most cost-effective strategy to manage this disease; however, durable resistance is difficult to achieve due to the vast virulence diversity of the anthracnose pathogen. Finding new genes with broad-spectrum resistance increases the prospect of designing an effective anthracnose-management strategy.

A Review of Angular Leaf Spot Resistance in Common Bean.

Angular leaf spot (ALS), caused by , is one of the most devastating diseases of common bean ( L.) in tropical and subtropical production areas. Breeding for ALS resistance is difficult due to the extensive virulence diversity of and the recurrent appearance of new virulent races.

Genome-Wide Linkage and Association Mapping of Halo Blight Resistance in Common Bean to Race 6 of the Globally Important Bacterial Pathogen.

pv. () Race 6 is a globally prevalent and broadly virulent bacterial pathogen with devastating impact causing halo blight of common bean ( L.).

High-resolution mapping reveals linkage between genes in common bean cultivar Ouro Negro conferring resistance to the rust, anthracnose, and angular leaf spot diseases.

Co-segregation analysis and high-throughput genotyping using SNP, SSR, and KASP markers demonstrated genetic linkage between Ur-14 and Co-3 /Phg-3 loci conferring resistance to the rust, anthracnose and angular leaf spot diseases of common bean. Rust, anthracnose, and angular leaf spot are major diseases of common bean in the Americas and Africa. The cultivar Ouro Negro has the Ur-14 gene that confers broad spectrum resistance to rust and the gene cluster Co-3 /Phg-3 containing two tightly linked genes conferring resistance to anthracnose and angular leaf spot, respectively.

Marker-Assisted Molecular Profiling, Deletion Mutant Analysis, and RNA-Seq Reveal a Disease Resistance Cluster Associated with Uromyces appendiculatus Infection in Common Bean Phaseolus vulgaris L.

Common bean ( L.) is an important legume, useful for its high protein and dietary fiber. The fungal pathogen (Pers.

Genetics and mapping of a new anthracnose resistance locus in Andean common bean Paloma.

Background: The Andean cultivar Paloma is resistant to Mesoamerican and Andean races of Colletotrichum lindemuthianum, the fungal pathogen that causes the destructive anthracnose disease in common bean. Remarkably, Paloma is resistant to Mesoamerican races 2047 and 3481, which are among the most virulent races of the anthracnose pathogen. Most genes conferring anthracnose resistance in common bean are overcome by these races.

Fine Mapping of , a Historically Important Rust Resistance Locus in Common Bean.

Bean rust, caused by , is a devastating disease of common bean () in the Americas and Africa. The historically important gene confers resistance to many races of the highly variable bean rust pathogen that overcome other rust resistance genes. Existing molecular markers tagging for use in marker-assisted selection produce false results.

Two endornaviruses show differential infection patterns between gene pools of Phaseolus vulgaris.

We investigated the occurrence of two plant endornaviruses, Phaseolus vulgaris endornavirus 1 and Phaseolus vulgaris endornavirus 2, in breeding lines, cultivars, landraces, and wild genotypes of common bean (Phaseolus vulgaris) collected from the two centers of common bean domestication: Mesoamerica and the Andes. The two endornaviruses were detected in many genotypes of Mesoamerican origin but rarely in genotypes of Andean origin. The results suggest that these two endornaviruses were introduced into the Mesoamerican modern genotypes during common bean domestication and provide more evidence for the existence of two divergent gene pools of common bean.

High-throughput SNP discovery and assay development in common bean.

Background: Next generation sequencing has significantly increased the speed at which single nucleotide polymorphisms (SNPs) can be discovered and subsequently used as molecular markers for research. Unfortunately, for species such as common bean (Phaseolus vulgaris L.) which do not have a whole genome sequence available, the use of next generation sequencing for SNP discovery is much more difficult and costly.

Variance in the chemical composition of dry beans determined from UV spectral fingerprints.

Nine varieties of dry beans representing five market classes were grown in three locations (Maryland, Michigan, and Nebraska), and subsamples were collected for each variety (row composites from each plot). Aqueous methanol extracts of ground beans were analyzed in triplicate by UV spectrophotometry. Analysis of variance-principal component analysis was used to quantify the relative variance arising from location, variety, between rows of plants, and analytical uncertainty and to test the significance of differences in the chemical composition.

The polyphenolic profiles of common bean ( L.).

Based on the phenolic profiles obtained by high performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-DAD-ESI/MS), 24 common bean samples, representing 17 varieties and 7 generic off-the-shelf items, belonging to ten US commercial market classes can be organized into six different groups. All of them contained the same hydroxycinnaminic acids, but the flavonoid components showed distinct differences. Black beans contained primarily the 3--glucosides of delphinidin, petunidin and malvidin, while pinto beans contained kaempferol and its 3--glycosides.

Occurrence and Distribution in Rwanda of Soilborne Fungi Pathogenic to the Common Bean.

The occurrence and distribution of soilborne fungi pathogenic to the common bean were determined by surveys conducted in seven of Rwanda's 10 prefectures during four growing seasons from 1989 to 1990. The pathogens were identified on the basis of symptoms, colony characteristics, reproductive structures, and pathogenicity tests. Of the plants sampled during the March through June 1989 season from four prefectures, 97% exhibited symptoms associated with soilborne pathogens.