Screening methylation of DNA repair genes in the oral mucosa of chronic smokers.

Objective: The aim of this study was to evaluate the epigenetic changes in the process of oral carcinogenesis by screening the methylation of repair genes in chronic smokers.

Design: Two groups were formed: Group 1: 16 smokers with consumption of 20 cigarettes/day for at least 10 years; and Group 2: 10 non-smoking. Exfoliative cytology of the tongue was performed, and the extracted DNA was treated by enzymes.

Initial oral biofilm formation on titanium implants with different surface treatments: An in vivo study.

Objective: The aim of this study was to examine in vivo the initial bacterial adhesion on titanium implants with different surface treatments.

Design: Ten subjects wore oral splints containing machined pure titanium disks (Ti-M), acid-etched titanium (Ti-AE) and anodized and laser irradiated disks (Ti-AL) for 24h. After this period, disks were removed from the splints and adherent bacteria were quantified by an enzymatic assay to assess total viable bacteria and by Real Time PCR to evaluate total bacteria and Streptococcus oralis levels.

Root surface treatment using diode laser in delayed tooth replantation: radiographic and histomorphometric analyses in rats.

Unlabelled: BACKGROUND AIM:  The aim of this study was to evaluate, by radiographic and histomorphometric analyses, the effects of high-power diode laser irradiation on the root surfaces of delayed replanted rat teeth.

Material And Methods:   Maxillary right incisors were extracted from 60 Wistar rats and kept dry for 60 min. Subsequently, the root canals were prepared and filled with calcium hydroxide paste.

Clinicopathologic and immunohistochemical features of oral neurofibroma.

Objective: The purposes of this study were to assess clinical, histopathological and immunohistochemical features of 22 oral neurofibromas (NFs) and discuss with previously described literature, addressing the main aspects regarding the differential diagnosis.

Materials And Methods: Immunohistochemical reactions included S-100, CD34, GLUT-1, EMA, Ki-67, p53 and Collagen IV and histochemical reactions for Alcian blue.

Results: Clinically, the preferential location was the maxillary bones, tongue and buccal mucosa.

Oral granuloma formation after injection of cosmetic filler.

The increased use of orofacial fillers in cosmetic procedures has led to new diagnostic challenges for dentists and oral pathologists. Here, we describe a case with multiple oral foreign body granulomas, which were formed after a polymethylmetacrylate injection for cosmetic purposes.

HOXB5 expression in oral squamous cell carcinoma.

Unlabelled: Human HOX genes encode transcription factors that act as master regulators of embryonic development. They are important in several processes such as cellular morphogenesis and differentiation. The HOXB5 gene in particular has been reported in some types of neoplasm, but not in oral cancer.

Diagnostic implications of oral intravascular papillary endothelial hyperplasia.

This study examined the clinical, histological, and immunohistochemical features as well as the differential diagnoses of oral intravascular papillary endothelial hyperplasia (IPEH) to aid clinicians and pathologists in its diagnosis. Clinical features of five oral IPEH cases were obtained from medical records, and all histopathological diagnoses were reviewed. Immunohistochemical reactions, including anti-CD-34, laminin, vimentin, estrogen receptor alpha, and Ki-67, were assessed.

Time- and concentration-dependent cytotoxicity of antibiotics used in endodontic therapy.

Objective: New drugs have to be assessed in endodontic therapy due to the presence of microorganisms resistant to therapeutic procedures. Thus, this study evaluated the time- and concentration-dependent cytotoxicity of different antibiotics used in endodontic therapy.

Material And Methods: Human gingival fibroblasts were treated and divided into the following experimental groups: Group I - control; Group II - ciprofloxacin hydrochloride; Group III - clyndamicin hydrochloride; and Group IV - metronidazole.

The neural histogenetic origin of the oral granular cell tumor: an immunohistochemical evidence.

Aims: Granular cell tumor (GCT) is a rare neoplasm that can appear in any site of the body, but most are located intraorally. Its histogenetic origin remains unclear. This report analyzes the immunoprofile of 15 cases of granular cell tumors, occurring in 13 women and 2 men and the lesions were located on the tongue or upper lip.

Giant cell fibroma of the maxillary gingiva in children: a case report.

The purpose of this paper is to describe a giant cell fibroma in the maxillary gingiva of an 11-year-old girl. After excisional biopsy, histological examination showed the presence of numerous giant, multinucleated, stellate-shaped cells dispersed throughout the fibrous tissue of the lamina propria. No recurrence was observed after the 1-year follow-up.

Interleukin-1 beta and interleukin-8 in healthy and inflamed dental pulps.

Unlabelled: After aggression to the dental pulp, some cells produce cytokines in order to start and control the inflammatory process. Among these cytokines, interleukin-1 beta (IL-1beta) and interleukin-8 (IL-8) emerge as important ones.

Objective: The purpose of this study was to analyze the location, distribution and concentration of these cytokines in healthy and inflamed dental pulps.

Intravascular papillary endothelial hyperplasia: report of 4 cases with immunohistochemical findings.

Intravascular papillary endothelial hyperplasia (IPEH) is a benign endothelial proliferation, usually intravascular, that may mimic angiosarcoma. In this report, four new cases of IPEH involving the oral region are described. The affected sites were the lower lip, labial comissure and the submandibular region.

Atypical presentation of oral basaloid squamous cell carcinoma.

Aim: The purpose of this report is to present the clinical and histological features of a basaloid squamous cell carcinoma (BSCC) occurring in the retromolar trigone of a 59-year-old man and to relate its immunohistochemical characteristics.

Background: BSCC is an aggressive distinct variant of squamous cell carcinoma (SCC) requiring recognition as a separate entity from SCC due to its peculiar behavior.

Case Report: A clinical examination revealed a 12x07x07 mm nodular mass with a rubbery consistency, defined borders, covered by reddish mucosa and an absence of bleeding upon palpation.

Occlusal changes in complete dentures processed by pack-and-press and injection-pressing techniques.

Ten pairs of stone casts were mounted in an articulator using a standardized maxillomandibular relation, on which trial dentures were constructed. Two groups were established: GI- pack-and-press technique; GII- injection-pressing technique. Intermaxillar contacts were marked and counted, and the contact between incisal pin and incisal table was measured, before and after the processing.