Publications by authors named "Marcia N C DA Cunha"
Article Synopsis
- The study optimized the production of collagenase and protease enzymes from Aspergillus heteromorphus URM0269 using submerged fermentation with soybean flour as the primary substrate, achieving maximum enzyme production under specific concentrations of substrate and yeast extract.
- After scaling up the fermentation process in a stirred tank bioreactor, collagenase activity significantly increased while protease activity decreased, highlighting a shift in enzyme production dynamics.
- The research found that collagenase functions best at 40°C and pH 6.0, indicating its potential use in biotechnology, and also provided thermodynamic parameters that suggest the feasibility of using soybean flour as a sustainable resource for enzyme production.
Article Synopsis
- Secretory immunoglobulin A (sIgA) is being explored for use in therapies related to the gut, with current research focused on its purification from Chinese hamster ovary (CHO) cell cultures.
- Researchers used aqueous two-phase systems (ATPS) in their study to purify sIgA monoclonal antibodies (mAbs), analyzing factors like pH and PEG concentration to optimize the process.
- The results indicated that under specific conditions, sIgA mAbs predominantly ended up in the PEG phase, and the method demonstrated potential for efficient and cost-effective manufacturing of sIgA biotherapeutics.
Purification and characterization of fibrinolytic protease from Streptomyces parvulus by polyethylene glycol-phosphate aqueous two-phase system.
Viviane N S Alencar Maria Clara DO Nascimento Julyanne V Dos Santos Ferreira Juanize M DA Silva Batista Marcia N C DA Cunha
An Acad Bras Cienc
December 2021
Fibrinolytic proteases are a promising alternative in the pharmaceutical industry, they are used in the treatment of cardiovascular diseases, especially thrombosis. Microorganisms are the most interesting source of fibrinolytic proteases. The aim of this study was the production of fibrinolytic protease from Streptomyces parvulus DPUA 1573, the recovery of the protease by aqueous two-phase system and partial biochemical characterization of the enzyme.
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