Introduction: The municipality of Jaciara was classified in 2003 as a transmission area for visceral leishmaniasis in outbreak situations. This study aimed to establish evidence of transmission of Leishmania (Leishmania) infantum chagasi by Lutzomyia cruzi in the municipality of Jaciara, State of Mato Grosso, Brazil.
Methods: This municipality is located 127 km from the state capital (Cuiabá) and is an important center for ecotourism practitioners.