Publications by authors named "Marcelo V Araujo"
Hepatic injuries in COVID-19 are not yet fully understood and indirect pathways (without viral replication in the liver) have been associated with the activation of vascular mechanisms of liver injury in humans infected with SARS-CoV-2. Golden Syrian hamsters are an effective model for experimental reproduction of moderate and self-limiting lung disease during SARS-CoV-2 infection. As observed in humans, this experimental model reproduces lesions of bronchointerstitial pneumonia and pulmonary vascular lesions, including endotheliitis (attachment of lymphoid cells to the luminal surface of endothelium).
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- * Lab tests on infected Golden Syrian hamsters showed that a sulfonated form of Calpeptin significantly reduced the viral load in the trachea when administered at a dose of 1 mg/kg body weight.
- * Targeting host proteins like cathepsins could provide a more stable treatment approach for COVID-19, despite a higher risk of side effects, compared to targeting the rapidly mutating virus itself.
Article Synopsis
- New variants of SARS-CoV-2 highlight the need for better vaccines, prompting the development of adenovirus-based vaccines that express the virus's Spike protein.
- The vaccine with the autophagy-inducing peptide C5 (HAd-Spike/C5) produced stronger cell-mediated immune responses and similar virus-neutralizing levels compared to the vaccine without it (HAd-Spike).
- In tests on golden Syrian hamsters, both vaccines showed effective protection against SARS-CoV-2, with HAd-Spike/C5 demonstrating slightly better outcomes in reducing illness and virus presence in the lungs.
Objectives: To develop alginate nanoparticles functionalized with polysorbate 80 (P80) as miltefosine carriers for brain targeting in the oral treatment of cryptococcal meningitis.
Methods: Miltefosine-loaded alginate nanoparticles functionalized or not with P80 were produced by an emulsification/external gelation method and the physicochemical characteristics were determined. The haemolytic activity and cytotoxic and antifungal effects of nanoparticles were assessed in an in vitro model of the blood-brain barrier (BBB).
Article Synopsis
- COVID-19 has led to over a million deaths globally, with significant gaps in understanding its neurological effects, despite known lung disease mechanisms.
- The study analyzed changes in brain cells (astrocytes) from Syrian hamsters infected with SARS-CoV-2, revealing disruptions in metabolic processes related to carbon metabolism and glycolysis, linked to neurological disorders.
- Findings indicate that SARS-CoV-2 affects brain regions like the hippocampus and cortex, potentially leading to neurological symptoms such as memory loss and cognitive impairment in infected individuals.
Therapeutic Vaccination with Cationic Liposomes Formulated with Dioctadecyldimethylammonium and Trehalose Dibehenate (CAF01) and Peptide P10 Is Protective in Mice Infected with .
J Fungi (Basel)
December 2020
The peptide P10 is a vaccine candidate for Paracoccidioidomycosis, a systemic mycosis caused by fungal species of the genus spp. We have previously shown that peptide P10 vaccination, in the presence of several different adjuvants, induced a protective cellular immune response mediated by CD4 Th lymphocytes that was associated with the increased production of IFN-γ in mice challenged with a virulent isolate of . Cationic liposomes formulated with dioctadecyldimethylammonium and trehalose dibehenate (DDA/TDB, termed also CAF01-cationic adjuvant formulation) have been developed for safe administration in humans and CAF01 liposomes are utilized as an adjuvant for modulating a robust Th/Th cellular response.
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