Publications by authors named "Marcelo Paulo Vaccari-Mazzetti"
Cleft lip and palate is a congenital malformation that requires a multidisciplinary treatment that evolves pediatrician, obstetrics, fetal medicine, genetics, plastic surgery, orthodontics, speech therapist, nursery, and psychology. Actually, the authors believe that it could be possible to ad protocols to use stem cells.The intrauterine diagnosis leads to preborn parental orientation and better parental collaboration to accept a precocious multidisciplinary treatment.
View Article and Find Full Text PDFBackground: Human cell cultures are being developed to replace various body tissues.
Objective: To assess the safety and efficacy of dermal regeneration with the injection of young autologous fibroblasts obtained from culture containing serum from patients themselves.
Materials And Methods: Dermal tissue from the groin of five patients was cultivated in M199 medium supplemented with 10% human serum.
Article Synopsis
- The study explores an experimental method to isolate and analyze mesenchymal stem cells from rabbit fat obtained through lipectomy, suggesting fat as a viable source for tissue repair.
- After surgically removing and processing fat from New Zealand rabbits, the researchers cultured the obtained cells, leading to successful growth and high viability rates.
- Results indicated that stem cell extraction using lipectomy is effective, yielding significant quantities of viable stem cells for potential bioengineering applications.
Purpose: To assess the technique for the collection of rabbit bone marrow stem cells from different regions to be used as an experimental model in regenerative medicine.
Methods: Thirty rabbits were allocated into 2 groups: GROUP A, n=8, animals that underwent bone marrow blood (BMB) harvesting from the iliac crest; and
Group B: including 22 rabbits that underwent BMB harvesting from the femur epiphysis. After harvesting, mononuclear cells were isolated by density gradient centrifugation (Ficoll - Histopaque).
Purpose: Evaluate by CT the use of porous vitreous carbon (PVC) and silicon (S) implants as the replacement bone in the craniofacial skeleton of rats.
Methods: 40 rats divided in: Group A (n=20) PVC submitted to the implant of a fragment in skull. After the euthanasia, the animals were divided into two subgroups: A I: 10 animals, studied in the 7th postoperative day (P.