Role of cardiac β-adrenergic and A-adenosine receptors in severe arrhythmias related to Parkinson's disease.

Aims: Although reduced life expectancy in Parkinson's Disease (PD) patients has been related to severe cardiac arrhythmias due to autonomic dysfunctions, its molecular mechanisms remain unclear. To investigate the role of cardiac β-Adrenergic (βAR) and A-Adenosine (AR) receptors in these dysfunctions, the pharmacological effects of stimulation of cardiac βAR (isoproterenol, ISO), in the absence and presence of cardiac βAR (atenolol, AT) or AR (1,3-dipropyl-8-cyclopentyl xanthine, DPCPX) blockade, on the arrhythmias induced by Ischemia/Reperfusion (CIR) in an animal PD model were studied.

Methods: PD was produced by dopaminergic lesions (confirmed by immunohistochemistry analysis) caused by the injection of 6-hydroxydopamine (6-OHDA, 6 μg) in rat striatum.

A Recombinant Chimeric Protein-Based Vaccine Containing T-Cell Epitopes from Amastigote Proteins and Combined with Distinct Adjuvants, Induces Immunogenicity and Protection against Infection.

Currently, there is no licensed vaccine to protect against human visceral leishmaniasis (VL), a potentially fatal disease caused by infection with Leishmania parasites. In the current study, a recombinant chimeric protein ChimT was developed based on T-cell epitopes identified from the immunogenic Leishmania amastigote proteins LiHyp1, LiHyV, LiHyC and LiHyG. ChimT was associated with the adjuvants saponin (Sap) or monophosphoryl lipid A (MPLA) and used to immunize mice, and their immunogenicity and protective efficacy were evaluated.

QUANTIFICATION OF NEURAL ELEMENTS IN POSTERIOR CRUCIATE LIGAMENT: COMPARISON BETWEEN HEALTHY KNEES AND WITH PRIMARY OSTEOARTHROSIS.

Objective: To quantify the neural elements in the posterior cruciate ligament (PCL) in healthy knees and with primary osteoarthrosis (OA).

Methods: In two groups with OA, one of cadavers and another of individuals, the area of neural elements identified in histological sections of PCL with anti-S100 immunohistochemistry was quantified.

Results: The overall mean area of the neural elements was 0.

Small Myristoylated Protein-3, Identified as a Potential Virulence Factor in Leishmania amazonensis, Proves to be a Protective Antigen against Visceral Leishmaniasis.

In a proteomics approach conducted with , parasite proteins showed either an increase or a decrease in their expression content during extensive in vitro cultivation, and were related to the survival and the infectivity of the parasites, respectively. In the current study, a computational screening was performed to predict virulence factors among these molecules. Three proteins were selected, one of which presented no homology to human proteins.

Effectiveness of histology and cytology on musculoskeletal tumor diagnosis.

Objective: To compare cytology and histology on the diagnosis of musculoskeletal neoplasms.

Method: Fifty eight cases available to evaluation were analyzed both by cytology and histology. The results of the biopsies studied by histology and cytology were compared to the results obtained on the surgical specimen or immunohistochemistry.

Neoplasm seeding in biopsy tract of the musculoskeletal system. A systematic review.

To identify, through a systematic literature review, the characteristics of neoplasm seeding in biopsy performed on the musculoskeletal system. We performed a search on PubMed, MEDLINE, LILACS and SciELO from August to October 2010. We included articles that addressed the neoplasm seeding in biopsy performed on the musculoskeletal system.

[Perceived distance to recreational facilities and the association with physical activity and exercise among adolescents in Curitiba, Paraná State, Brazil].

The aim of this study was to verify the association between perceived distance to recreational facilities (parks, gyms, sports courts, and bike lanes) for physical activity and exercise among adolescents in Curitiba, Paraná State, Brazil. A cross-sectional study was conducted with a representative sample of 1,474 adolescents (14-18 years) enrolled in public schools. There was an inverse association between distance (> 31 minutes) to gyms and sports courts and physical activity among boys (PR = 0.

TUMOR CONTAMINATION IN THE BIOPSY PATH OF PRIMARY MALIGNANT BONE TUMORS.

Objective: To study factors possibly associated with tumor contamination in the biopsy path of primary malignant bone tumors.

Method: Thirty-five patients who underwent surgical treatment with diagnoses of osteosarcoma, Ewing's tumor and chondrosarcoma were studied retrospectively. The sample was analyzed to characterize the biopsy technique used, histological type of the tumor, neoadjuvant chemotherapy used, local recurrences and tumor contamination in the biopsy path.

QUALITY OF LIFE AND SOCIAL CHARACTERISTICS OF PATIENTS SUBMITTED TO TOTAL KNEE ARTHROPLASTY.

Objective: To study the quality of life (QL) and social characteristics of patients submitted to total knee arthroplasty (TKA).

Methods: The Brazilian validated version of the SF-36 Quality of Life Questionnaire was applied during the pre- and postoperative periods to assess the effect of TKA in QL. A semi-structured social questionnaire was used for the survey of patients' socioeconomic profile.

The role of cytology in the diagnosis of musculoskeletal neoplasms: systematic review.

The authors systematically reviewed the literature of the last decade on the role of cytology in the evaluation of musculoskeletal neoplasms, and its diagnostic accuracy. A search was carried out on the databases PubMed, MEDLINE, LILACS and SciELO, selecting articles in which cytology was used in the diagnosis of musculoskeletal neoplasms. Limits were used for English, Spanish and Portuguese, and only articles published since 2000 were selected.