A tomato B-box protein regulates plant development and fruit quality through the interaction with PIF4, HY5, and RIN transcription factors.

During the last decade, knowledge about BBX proteins has greatly increased. Genome-wide studies identified the BBX gene family in several ornamental, industry, and food crops; however, reports regarding the role of these genes as regulators of agronomically important traits are scarce. Here, by phenotyping a knockout mutant, we performed a comprehensive functional characterization of the tomato locus Solyc12g089240, hereafter called SlBBX20.

A potential antiviral against COVID-19 obtained from Byrsonima coccolobifolia leaves extract.

In this study, we specifically focused on the crude methanolic leaf extract of Byrsonima coccolobifolia, investigating its antifungal potential against human pathogenic fungi and its antiviral activity against COVID-19. Through the use of high-performance liquid chromatography coupled with electrospray ionization ion trap tandem mass spectrometry, direct infusion electrospray ionization ion trap tandem mass spectrometry, and chromatographic dereplication procedures, we identified galloyl quinic acid derivatives, catechin derivatives, proanthocyanidins, and flavonoid glycosides. The broth dilution assay revealed that the methanolic leaf extract of B.

Inaugural Description of Extrafloral Nectaries in Sapindaceae: Structure, Diversity and Nectar Composition.

Sapindales is a large order with a great diversity of nectaries; however, to date, there is no information about extrafloral nectaries (EFN) in Sapindaceae, except recent topological and morphological data, which indicate an unexpected structural novelty for the family. Therefore, the goal of this study was to describe the EFN in Sapindaceae for the first time and to investigate its structure and nectar composition. Shoots and young leaves of were fixed for structural analyses of the nectaries using light and scanning electron microscopy.

Lipopeptides from an isolate of Bacillus subtilis complex have inhibitory and antibiofilm effects on Fusarium solani.

Bacillus subtilis species complex is known as lipopeptide-producer with biotechnological potential for pharmaceutical developments. This study aimed to identify lipopeptides from a bacterial isolate and evaluate their antifungal effects. Here, we isolated and identified a lipopeptide-producing bacterium as a species of Bacillus subtilis complex (strain UL-1).

Chemical Constituents from Leaves of (Asteraceae) and Their Antioxidant Effects.

is one of the largest genera of Asteraceae and its species are used in folk medicine for several medicinal purposes due to the presence of bioactive compounds. We investigated the phytochemical composition of polar extracts of . Using chromatographic procedures, diterpenoids (-kaurenoic acid), flavonoids (hispidulin, eupafolin, isoquercitrin, quercitrin, biorobin, rutin, and vicenin-2), caffeic acid, and chlorogenic acid derivatives (5--caffeoylquinic acid and its methyl ester, 3,4-di--caffeoylquinic acid, 4,5-di--caffeoylquinic acid, and 3,5-di--caffeoylquinic acid and its methyl ester) were isolated from polar fractions and are described.

Dereplication of Cytochalasans and Octaketides in Cytotoxic Extracts of Endophytic Fungi from (Salicaceae).

Endophytes have been shown to be a source of novel drug prototypes. The genus is known for presenting cytotoxic clerodane diterpenes; however, there are few reports on secondary metabolites produced by its fungal microbiota. Thus, in the present study endophytic fungi obtained from the fresh leaves of were grown in potato dextrose broth and rice to perform a secondary metabolite prospection study.

Antioxidant Leaf Extract Suppresses Lipid Accumulation in Dependent on Transcription Factor NHR-49.

Obesity is a global public health problem that is associated with oxidative stress. One of the strategies for the treatment of obesity is the use of drugs; however, these are expensive and have numerous side effects. Therefore, the search for new alternatives is necessary.

Genome mining of sp. BRB081 reveals the production of the antitumor pyrrolobenzodiazepine sibiromycin.

Unlabelled: Employing a genome mining approach, this work aimed to further explore the secondary metabolism associated genes of sp. BRB081, a marine isolate. The genomic DNA of BRB081 was sequenced and assembled in a synteny-based pipeline for biosynthetic gene clusters (BGCs) annotation.

Galloylquinic acid derivatives from Byrsonima fagifolia leaf extract and potential antifungal activity.

Ethnopharmacological Relevance: Byrsonima fagifolia Niedenzu (Malpighiaceae) and other Byrsonima species are popularly employed in Brazilian traditional medicine in the form of preparations as cicatrizing, anti-inflammatory, and antimicrobial.

Aim Of The Study: To characterize the phytochemical profile of the hydromethanolic extract obtained from B. fagifolia leaves (BF extract) and to evaluate the toxicity and the antifungal activity.

OSMAC Strategy Integrated with Molecular Networking for Accessing Griseofulvin Derivatives from Endophytic Fungi of (Asteraceae).

Three endophytic fungi isolated from (Less.) G. Sancho (Asteraceae) were cultivated using the one strain many compounds (OSMAC) strategy to evaluate the production of griseofulvin derivatives.

Two Origins, Two Functions: The Discovery of Distinct Secretory Ducts Formed during the Primary and Secondary Growth in .

Secretory ducts have been reported for more than 50 families of vascular plants among primary and secondary tissues. A priori, all ducts of a plant are of the same type, and only slight variations in the concentration of their compounds have been reported for few species. However, two types of secretion were observed in primary and secondary tissues of , leading us to investigate the possible influence of duct origins on the structure and metabolism of this gland.

Identification of flavonoid-3-O-glycosides from leaves of Casearia arborea (Salicaceae) by UHPLC-DAD-ESI-HRMS/MS combined with molecular networking and NMR.

Introduction: Casearia is an essential source of cytotoxic highly oxidised clerodane diterpenes, in addition to phenolics, flavonoids, and glycoside derivatives. Here we identify flavonoid-3-O-glycoside derivatives in the ethyl acetate (EtOAc) fraction of the methanolic extract from leaves C. arborea leaves.

UV-B and Drought Stress Influenced Growth and Cellular Compounds of Two Cultivars of Phaseolus vulgaris L. (Fabaceae).

Combined enhanced UV-B radiation and drought may induce different morphological and physiological alterations in plants than either abiotic stress alone. We evaluated morphology, biomass, and primary and secondary metabolism changes in seedlings of two common bean cultivars, IAC Imperador (drought-resistant) and IAC Milênio. To test the hypothesis that cultivars responded differently to combined stresses in a controlled environment, seedlings of the examined been cultivars were exposed to UV-B and/or drought treatments for three weeks.

Sesquiterpene Lactones from : Absolute Configuration and Structural Requirements for Antitumor Activity.

This work describes the chromatographic fractionation of the aerial parts of and the structural characterization and determination of the absolute configuration of the isolated compounds as well as their antitumor potential. The HPLC fractionation of the CHCl phase of the MeOH extract from the leaves of led to the isolation of two related sesquiterpene lactones (STLs): calein C () and calealactone B (). Additionally, during the purification process, a derivative of calein C () was formed as a product of the Michael addition of MeOH.

Bioaffinity Fishing Procedure Using Secretory Phospholipase A2 for Screening for Bioactive Components: Modulation of Pharmacological Effect Induced by sPLA2 from by Hispidulin from .

Bioaffinity capturing of molecules allows the discovery of bioactive compounds and decreases the need for various stages in the natural compound isolation process. Despite the high selectivity of this technique, the screening and identification methodology depends on the presence of a protein to capture potential ligands. However, some proteins, such as snake secretory phospholipase A2 (sPLA2), have never been investigated using this approach.

Marine Bacteria from Rocas Atoll as a Rich Source of Pharmacologically Active Compounds.

Rocas Atoll is a unique environment in the equatorial Atlantic Ocean, hosting a large number of endemic species, however, studies on the chemical diversity emerging from this biota are rather scarce. Therefore, the present work aims to assess the metabolomic diversity and pharmacological potential of the microbiota from Rocas Atoll. A total of 76 bacteria were isolated and cultured in liquid culture media to obtain crude extracts.

Two New Flavonoids from the Leaves of (Ruiz and Pav.) Pers. (Asteraceae).

In this work, two new flavonoids, oblongifolioside A () and oblongifolioside B (), along with eight known compounds (-), are isolated from the leaves of (Asteraceae). The new structures are established through spectroscopic data and the known compounds are identified by comparison with data reported in the literature. The compounds (-) are evaluated in relation to their antiradical properties.

Evaluation of the Inhibitory Potential of Casuarictin, an Ellagitannin Isolated from White Mangrove () Leaves, on Snake Venom Secretory Phospholipase A2.

Ellagitannins constitute the largest group of hydrolyzable tannins of plants, and, from this group, casuarictin (Casu) was identified in some plant species. However, to our knowledge, no investigation of secretory phospholipase A2 (sPLA2) inhibition by Casu has been performed yet. Casuarictin was isolated by chromatography n-butanol (n-BuOH) partition of leaves.

Acylated Flavonoid Glycosides are the Main Pigments that Determine the Flower Colour of the Brazilian Native Tree (Cham.) Cogn.

(Cham.) Cogn. is a plant native to Brazil whose genus and family (Melastomataceae) are poorly studied with regards to its metabolite profile.

Calein C, a Sesquiterpene Lactone Isolated From (), Inhibits Mitotic Progression and Induces Apoptosis in MCF-7 Cells.

Breast cancer is the most common cancer in women worldwide. Estrogen receptor-positive (ER+) breast cancer represents approximately 75% of diagnosed cases, while 15-20% of them are triple-negative (TN). Although there have been improvements in the therapeutic approach, the mortality rate remains elevated.

Osmophores and floral fragrance in and (Anacardiaceae): an overlooked secretory structure in Sapindales.

Flowers of Anacardiaceae and other Sapindales typically produce nectar, but scent, often associated with a reward for pollinators, has surprisingly been mentioned only rarely for members of the family and order. However, flowers of and produce a strong sweet scent. The origin and composition of these floral scents is the subject of this study.

Antileishmanial activity and ultrastructural changes of sesquiterpene lactones isolated from Calea pinnatifida (Asteraceae).

Bioactivity-guided fractionation of antileishmanial active CHCl phase of MeOH extract from leaves of Calea pinnatifida led to isolation of two sesquiterpene lactones calein C (1) and calealactone C (2), which structures were stablished on the basis of spectroscopic analysis. Compounds 1 and 2 displayed potent activity against Leishmania amazonensis promastigotes with EC of 1.7 and 4.

Non-Clinical Studies for Evaluation of 8-C-Rhamnosyl Apigenin Purified from Peperomia obtusifolia against Acute Edema.

Compound 8--rhamnosyl apigenin (8CR) induced a moderate reduction in the enzymatic activity of secretory phospholipase A2 (sPLA2) from and cytosolic phospholipase A2 (cPLA2), but the compound also significantly inhibited the enzymatic activity of the enzyme cyclooxygenase. In vitro assays showed that the compound induced a slight change in the secondary structure of sPLA2 from snake venom. In vivo assays were divided into two steps.

Evaluation of Potential Thrombin Inhibitors from the White Mangrove (Laguncularia racemosa (L.) C.F. Gaertn.).

The aim of this work was to verify the effects of methanol (MeOH) and hydroalcoholic (HA) extracts and their respective partition phases obtained from white mangrove (Laguncularia racemosa (L.) C.F.

Antiproliferative activity of flavonoids from Croton sphaerogynus Baill. (Euphorbiaceae).

Croton sphaerogynus is a shrub from the Atlantic Rain Forest in southeastern Brazil. A lyophilized crude EtOH extract from leaves of C. sphaerogynus, obtained by maceration at room temperature (seven days), was suspended in methanol and partitioned with hexane.