A Combination of Artificial Intelligence with Genetic Algorithms on Static Time-Lapse Images Improves Consistency in Blastocyst Assessment, An Interpretable Tool to Automate Human Embryo Evaluation: A Retrospective Cohort Study.

Background: In recent times, various algorithms have been developed to assist in the selection of embryos for transfer based on artificial intelligence (AI). Nevertheless, the majority of AI models employed in this context were characterized by a lack of transparency. To address these concerns, we aim to design an interpretable tool to automate human embryo evaluation by combining artificial neural networks (ANNs) and genetic algorithms (GA).

Modulating the lipid profile of blastocyst cell membrane with DPPC multilamellar vesicles.

The aim of this study was to evaluate the effect of multilamellar vesicles (MLVs) of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) in co-culture with -produced bovine embryos (IVPEs). The stability of five concentrations of MLVs (1.0, 1.

Paternal effect does not affect in vitro embryo morphokinetics but modulates molecular profile.

The use of different sires influences in vitro embryo production (IVP) outcome. Paternal effects are observed from the first cleavages until after embryonic genome activation (EGA). Little is known about the mechanisms that promote in vitro fertility differences, even less about the consequences on embryo development.

Influence of cAMP modulator supplementation of in vitro culture medium on Bos taurus indicus embryos.

The effectiveness of the use of natriuretic peptide C (NPPC) in the blocking of meiosis has already been proven in several species. However, there are no reports on the use of NPPC in the activation of metabolic processes in embryos. Whereas modulations of cAMP concentrations alter the lipid metabolism of bovine oocytes, the present study aims to evaluate the effect of NPPC on the development, lipid content and transcript levels of genes related to lipid metabolism of IVP bovine embryos.

Extracellular vesicles of follicular fluid from heat-stressed cows modify the gene expression of in vitro-matured oocytes.

The effect of heat stress (HS) on cattle reproduction is deleterious with respect to ovarian follicular development and oocyte quality. The objective of this study was to investigate the effect of follicular fluid extracellular vesicles (EVs) obtained from cows maintained in thermoneutral (TN) or HS conditions on in vitro oocyte maturation. Nonlactating cows were estrous synchronized.

Artificial Intelligence-Based Grading Quality of Bovine Blastocyst Digital Images: Direct Capture with Juxtaposed Lenses of Smartphone Camera and Stereomicroscope Ocular Lens.

In this study, we developed an online graphical and intuitive interface connected to a server aiming to facilitate professional access worldwide to those facing problems with bovine blastocysts classification. The interface Blasto3Q, where 3Q refers to the three qualities of the blastocyst grading, contains a description of 24 variables that were extracted from the image of the blastocyst and analyzed by three Artificial Neural Networks (ANNs) that classify the same loaded image. The same embryo (i.

Corrigendum to "Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on Produced Embryos".

[This corrects the article DOI: 10.1155/2017/1502489.].

Heat shock proteins (HSP): dermatological implications and perspectives.

In recent years, several studies have demonstrated the protective effect of Heat Shock Proteins (HSP) on different organs and tissues under stressful conditions. However, most research explores the performance of those molecular chaperones during immune responses or pathological conditions like cancer, whereas the number of studies related to the performance of HSPs in the skin during diverse natural or physiopathological conditions is very low. Therefore, the aim of this article was to summarize the main concepts concerning the expression and performance of HSPs, from analysis of current medicine and cosmetics publications, as well as exploring the importance of these proteins in the dermatological area in physiological events such as cutaneous aging, skin cancer and wound healing and to present final considerations related to biotechnology performance in this area.

The effect of type of vaginal insert and dose of pLH on embryo production, following fixed-time AI in a progestin-based superstimulatory protocol in Nelore cattle.

The objective was to analyze and report field data focusing on the effect of type of progesterone-releasing vaginal insert and dose of pLH on embryo production, following a superstimulatory protocol involving fixed-time artificial insemination (FTAI) in Nelore cattle (Bos taurus indicus). Donor heifers and cows (n = 68; 136 superstimulations over 2 years) received an intravaginal, progesterone-releasing insert (CIDR or DIB, with 1.9 or 1.

Do high progesterone concentrations decrease pregnancy rates in embryo recipients synchronized with PGF2alpha and eCG?

The objective of this study was to evaluate the effects of equine chorionic gonadotropin (eCG) treatment on the number of induced accessory corpora lutea (CL), plasma progesterone concentrations and pregnancy rate in cross-bred heifers after transfer of frozen-thawed (1.5M ethylene glycol) embryos. All recipients received 500 microg PGF2alpha (dl-cloprostenol, i.

Embryo recovery and pregnancy rates after the delay of ovulation and fixed time insemination in superstimulated beef cows.

The aim of this study was to evaluate the effect of delaying ovulation subsequent to superstimulation of follicular growth in beef cows (Bos indicus) on embryo recovery rates and the capacity of embryos to establish pregnancies. Ovulation was delayed by three treatments using either progesterone (CIDR-B) or a GnRH agonist (deslorelin). Multiparous Nelore cows (n = 24) received three of four superstimulation treatments in an incomplete block design (n = 18 per group).