Comparison of in vitro chondrogenic potential of human mesenchymal stem cells derived from bone marrow and adipose tissue.

In the present work, the human bone marrow and adipose tissue-derived mesenchymal stem cells (MSCs) were isolated and expanded under in vitro condition. After their phenotypic analysis, the chondrogenic differentiation was induced by using of the three-dimensional culture system without supplementation of growth factors, and their chondrogenic potential was compared. Obtained results proved that both types of MSCs undergo the process of chondrogenic differentiation.

Morphology of in vitro expanded human muscle-derived stem cells.

Background: Skeletal muscle contains populations of multipotent adult stem cells also referred to as muscle-derived stem cells.

Aim: The main goal of this study was to isolate and culture human adult stem cells from skeletal muscle and characterize them.

Methods: Muscle-derived stem cells were isolated from biopsy specimens of femoral muscle.

Autologous biograft and mesenchymal stem cells in treatment of the diabetic foot.

Objectives: This study was performed to test a new technique for treatment of chronic non-healing wound (diabetic ulcer) using autologous biograft composed of autologous skin fibroblasts on biodegradable collagen membrane (Coladerm) in combination with autologous mesenchymal stem cells (MSC) derived from the patient's bone marrow.

Design: The bone marrow aspirate of the patient with diabetic foot was applied directly to the wound and injected into the edges of the wound, finally covered with prepared autologous biograft. The patient received two additional treatments with cultured MSC on day 7 and 17.