Whole-genome sequencing-based characterization of sp. 6(4): focus on natural product.

We have sequenced the whole genome of sp. 6(4) isolated from tomato roots that presents antifungal activity against phytopathogenic fungi, mainly . The genome has almost 7 Mb and 3368 hypothetical proteins that were analysed and characterized in Uniprot with the emphasis on biological compounds.

The intricate molecular identification of Streptomyces: a case study on Antarctic soil isolates.

Despite the worldwide use of 16S rRNA to identify bacterial species, the use of this gene does not discriminate the 750 species in the genus Streptomyces. A MLST scheme was constructed with rpoB, gyrB, recA, trpB and atpD genes to access the genomic variances in Streptomyces species evolution. We analyze the housekeeping genes in 49 Streptomyces isolates from Antarctic soil.

Evaluation of BOX-PCR and REP-PCR as Molecular Typing Tools for Antarctic Streptomyces.

Molecular studies led to the resurgence of natural products research from genus Streptomyces, already known for their long history and importance for the pharmaceutical industry. However, species belonging to this genus are difficult to identify and the most commonly used techniques, which are based on 16S rRNA sequencing, do not discriminate between related species. In this work, amplification profiles generated from BOX-PCR and REP-PCR of 49 Antarctic soil streptomycetes were compared to evaluate the diversity present in the group and to characterize the bacterial isolates, along with some 16S rRNA amplifications.