Effect of loop-grafting on the activity, stability and regioselectivity of Priestia megaterium levansucrase using two inulosucrases as loop donors.

Active-site loops of enzymes modulate activity, stability, regioselectivity and substrate specificity. This study examines the impact of loop transplantation on Priestia megaterium levansucrase Pm-SacB, which produces levan-type, β-(2,6)-linked fructooligosaccharides. Inulosucrases from Lactobacillus johnsonii (Lj-InuJ) and Halalkalicoccus jeotgali (Hje-Inu), which synthesize β-(2,1)-linked inulin fructans, served as donors of eight active-site loops.

Modifying the Glycocalyx of Melanoma Cells via Metabolic Glycoengineering Using -Acetyl-d-glucosamine Analogues.

Tumor cells are decorated with aberrant glycan structures on cell surfaces. It is well known that the glycocalyx serves as a main cellular regulator, although its role in cancer is still not completely understood. Over recent decades, several non-natural monosaccharides carrying clickable groups have been introduced in melanoma cells.

Identification of mureidomycin analogues and functional analysis of an N-acetyltransferase in napsamycin biosynthesis.

Antibiotic abundance: Several new uridyl peptide antibiotics were identified from a heterologous producer strain containing the mureidomycin/napsamycin biosynthetic gene cluster by using HRMS and LC-ESI-MS/MS. Analysis of the new compounds and the corresponding gene cluster revealed NpsB, an N-acetyltransferase, to be responsible for acetylation of the uridyl peptide antibiotic.