Publications by authors named "Marc Pages Gallego"
Background: Nanopore-based DNA sequencing relies on basecalling the electric current signal. Basecalling requires neural networks to achieve competitive accuracies. To improve sequencing accuracy further, new models are continuously proposed with new architectures.
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- - The study highlights the role of the cellular environment alongside genetic factors in the onset and progression of diseases, emphasizing that different cell types have unique extracellular needs that influence disease outcomes.
- - It focuses on hsa-microRNA-31-5p (miR-31), which is overexpressed in keratinocytes in psoriatic skin, revealing that its expression increases under low glucose conditions and enhances cell survival by promoting glutamine metabolism.
- - The research demonstrates that miR-31 causes the secretion of specific metabolites and immunomodulatory factors that stimulate Th17 cell differentiation, crucial for psoriasis; targeting this metabolic pathway offers potential treatment strategies as shown in a mouse model.
p53 Forms Redox-Dependent Protein-Protein Interactions through Cysteine 277.
Antioxidants (Basel)
October 2021
Article Synopsis
- - Reversible cysteine oxidation influences protein structure and function, enhancing redox signaling by enabling disulfide bond formation that stabilizes protein interactions under oxidative stress.
- - The tumor suppressor protein p53 is redox-sensitive and undergoes reversible cysteine oxidation, but the specifics of its interactions with other proteins in oxidative conditions remain unclear.
- - Recent research shows that p53 forms disulfide bonds with several interacting proteins in oxidizing environments, with cysteine 277 being critical for these interactions, potentially modulating p53's activity.
Redox signaling is controlled by the reversible oxidation of cysteine thiols, a post-translational modification triggered by HO acting as a second messenger. However, HO actually reacts poorly with most cysteine thiols and it is not clear how HO discriminates between cysteines to trigger appropriate signaling cascades in the presence of dedicated HO scavengers like peroxiredoxins (PRDXs). It was recently suggested that peroxiredoxins act as peroxidases and facilitate HO-dependent oxidation of redox-regulated proteins via disulfide exchange reactions.
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- The study examines how WNT and Notch signaling influence the differentiation of intestinal stem cells (CBCs) into various cell types, particularly noting a metabolic transition towards lower mitochondrial activity during this process.
- It highlights the role of Forkhead box O (FoxO) transcription factors in regulating CBCs, showing that deletion of FoxO genes in mice leads to increased differentiation into secretory cells.
- The research indicates that FoxO and Notch pathways interact to control mitochondrial fission, which is crucial for determining the fate of stem cells and their differentiation into specific intestinal cell types such as goblet cells and Paneth cells.
Symmetry breaking is an essential step in cell differentiation and early embryonic development. However, the molecular cues that trigger symmetry breaking remain largely unknown. Here, we show that mitochondrial HO acts as a symmetry-breaking cue in the C.
View Article and Find Full Text PDFIn eukaryotes, nucleosomes form a barrier to DNA templated reactions and must be dynamically disrupted to provide access to the genome. During nucleosome (re)assembly, histones can be replaced by new histones, erasing post-translational modifications. Measuring histone turnover in mammalian cells has mostly relied on inducible overexpression of histones, which may influence and distort natural histone deposition rates.
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