Evaluation of capillary zone electrophoresis for charge heterogeneity testing of monoclonal antibodies.

Within pharmaceutical industry charge heterogeneity testing of biopharmaceuticals has to be reproducible and fast. It should pass method validation according to ICH Q2. Classical approaches for the analysis of the charge heterogeneity of biopharmaceuticals are ion exchange chromatography (IEC) and isoelectric focusing (IEF).

Influence of solvent properties on separation and detection performance in non-aqueous capillary electrophoresis-mass spectrometry of basic analytes.

The versatility of non-aqueous capillary electrophoresis (NACE) results mainly from the variety of physico-chemical properties of the different solvents. They provide solubility for a wide range of analytes, enable to control electrophoretic selectivity, but affect in some cases UV absorbance detection. The coupling of NACE to electrospray mass spectrometry (ESI-MS) allows to cope with the high UV cut-off of some CE relevant solvents (e.

Development of a new approach for screening combinatorial libraries using MALDI-TOF-MS and HPLC-ESI-MS/MS.

Mass spectrometric techniques play a prominent role in the rapidly expanding field of high-throughput screening (HTS). In this paper, the authors present a novel qualitative approach for the screening of a small library of compounds using MALDI-TOF-MS and HPLC-ESI-MS/MS. Chymotrypsin (CT), a serine protease, was selected as the target protein.

Control of electroosmotic flow in nonaqueous capillary electrophoresis by polymer capillary coatings.

In aqueous capillary electrophoresis the electroosmotic flow (EOF) can be strongly suppressed or eliminated by coating the capillary surface silanols either by buffer additive adsorption or chemical modification. Hydrophilic coatings, e.g.