BACH2 enforces the transcriptional and epigenetic programs of stem-like CD8 T cells.

During chronic infection and cancer, a self-renewing CD8 T cell subset maintains long-term immunity and is critical to the effectiveness of immunotherapy. These stem-like CD8 T cells diverge from other CD8 subsets early after chronic viral infection. However, pathways guarding stem-like CD8 T cells against terminal exhaustion remain unclear.

A Genetic Screen Reveals that Synthesis of 1,4-Dihydroxy-2-Naphthoate (DHNA), but Not Full-Length Menaquinone, Is Required for Cytosolic Survival.

Through unknown mechanisms, the host cytosol restricts bacterial colonization; therefore, only professional cytosolic pathogens are adapted to colonize this host environment. is a Gram-positive intracellular pathogen that is highly adapted to colonize the cytosol of both phagocytic and nonphagocytic cells. To identify determinants of cytosolic survival, we designed and executed a novel screen to isolate mutants with cytosolic survival defects.