Several naturally occurring polysaccharides were purified and subsequently sulfated by chlorosulfonic acid-pyridine complex. These were isolated as the sodium salt and further purified by ECTEOLA cellulose chromatography. Anticoagulant properties of the sulfated polysaccharides were compared with commercial heparin by measuring their in vitro effects on activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT) using pooled normal human plasma.
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