Cooling and Cryopreservation of Equine Platelet-Rich Plasma With Dimethyl Sulfoxide and Trehalose.

Equine platelet-rich plasma (PRP) has been used in horses to repair bone, articular and tendinous lesions, laminitis, and even endometritis. However, platelets have a very limited lifespan, which makes it difficult to prepare and use PRP, except in loco. With the aim to produce PRP with higher platelet viability for clinical purposes, the effects of the cryoprotectants dimethyl sulfoxide (DMSO) and trehalose were evaluated on cooled (4°C) and cryopreserved (-196°C) equine PRP.

Resynchronization improves reproductive efficiency of suckled Bos taurus beef cows subjected to spring-summer or autumn-winter breeding season in South Brazil.

The objective of the present study was to evaluate the effect of estrus resynchronization (RE) after the first Timed Artificial Insemination (TAI) protocol on reproductive efficiency of suckled Bos taurus beef cows (n = 1052; Angus and Brangus) subjected to two different breeding season (BS) in Rio Grande do Sul, Brazil. In experiment 1, cows were maintained in native pasture on conventional BS (spring-summer), and in experiment 2, cows were maintained in a grazing area cultivated in an integrated crop-livestock system on modified BS (autumn -winter). Experiment 1 cows (n = 92) were randomly distributed in two reproductive managements: 1) TAI at the first day of the BS (Day 0), followed by natural service (NS) on day 10 until the end of the BS (TAI + NS; n = 45) or, 2) TAI on day 0, followed by RE 22 days after the first TAI and then followed by NS on day 42 until the end of the BS (TAI + RE22 + NS; n = 47).

Impact of spontaneous Neospora caninum infection on pregnancy loss and subsequent pregnancy in grazing lactating dairy cows.

The impact of spontaneous Neospora caninum infection on pregnancy loss and subsequent pregnancy in grazing lactating dairy cows was evaluated. Data from 1273 females (878 multiparous and 395 first-calving cows) from six preselected dairy herds were analyzed. Cows were classified as seropositive (SP) (prevalence, 24%; range, 11%-33%) or seronegative (SN) by indirect immunofluorescence detection of antibodies against N caninum.