The Rhyacophila fasciata Species Complex (Trichoptera: Rhyacophilidae) in Turkey with description of a new species, Rhyacophila anatolica Ekingen & Valladolid, sp. nov., based on morphological, genetic, and ecological evidence.

The morphology of the larva, pupa, male, and female of Rhyacophila anatolica Ekingen & Valladolid, sp. nov., from Turkey is described.

A histological procedure for the study of insect chitinized tissues in light microscopy.

We present a procedure to obtain histological preparations of insect cuticles for their observation in ligth microscopy. The use of EDTA (ethylendiaminetetraacetic acid), as a chelator of certain metals present in chitin, soften the tissues and allows us to cut them. The tissues were embbeding in plastic resines in two different protocols and cutted by cryostat (20-30 μm) and ultramicrotome (4 μm) respectively.

The Rhyacophila fasciata Species Complex (Trichoptera: Rhyacophilidae) in Central Europe with description of a new species, Rhyacophila loeffleri Valladolid & Waringer, n. sp., based on morphological, genetic and ecological evidence.

The morphology of the larva, pupa, male, and female of Rhyacophila loeffleri Valladolid & Waringer, n. sp., from Austria, Czech Republic, France, and Italy is described.

The Rhyacophila fasciata Group in Europe: Rhyacophila macedonica Karaouzas, Valladolid amp; Ibrahimi (n. sp.) from Greece, Kosovo, Republic of North Macedonia and Serbia (Trichoptera: Rhyacophilidae).

The morphology of all postembryonic stages (larva, pupa, male, and female) of Rhyacophila macedonica Karaouzas, Valladolid, Ibrahimi n. sp. from Greece, Republic of North Macedonia, Kosovo and Serbia was examined.

Would delineation of nitrate vulnerable zones be improved by introducing a new parameter representing the risk associated with soil permeability in the Land Use-Intrinsic Vulnerability Procedure?

Most methods for mapping groundwater vulnerability are based on the excessively simplistic approach that aquifer recharge is produced by vertical infiltration. The novel Land Use-Intrinsic Vulnerability (LU-IV) procedure assesses groundwater vulnerability to nitrate pollution over the entire territory, including aquifers catchment areas. In this research, it was analysed if the delineation of nitrate vulnerable zones (NVZs) would be improved by introducing a new parameter representing the risk associated with soil permeability (parameter S) in the procedure.

N and P behaviour in alluvial aquifers and in the soil solution of their catchment areas: How land use and the physical environment contribute to diffuse pollution.

The role of land use and the physical environment in N and P pollution of alluvial aquifers was analysed at three levels of information: (1) aquifer (N and P in groundwater), (2) soil transect (potentially leachable N and P in the soil solution) and (3) aquifer's catchment area. The study was carried out in the Oja and Tirón alluvial aquifers and their catchment areas (northern Spain). Nitrate was the dominant N form, both in groundwater and the soil solution of aquifers' catchment areas.

The Rhyacophila fasciata Group in Europe: Rhyacophila fasciata Hagen 1859 and formerly synonymized species (Trichoptera: Rhyacophilidae), with new description of Rhyacophila fasciata and Rhyacophila septentrionis McLachlan 1865 (stat. prom.).

The presence and distribution of Rhyacophila fasciata Hagen 1859 in Europe were revised, based on bibliographic study, collection specimens, and new material collected in different countries. The status of formerly synonymized species, Rhyacophila ferruginea (Scopoli 1763) and Rhyacophila septentrionis McLachlan 1865 was also assessed. The type of R.

The Rhyacophila fasciata Group in Croatia and Bosnia and Herzegovina: Rhyacophila f. fasciata Hagen 1859 and the description of two new subspecies, Rhyacophila fasciata delici Kučinić Valladolid (ssp. nov.) from Croatia and Bosnia and Herzegovina and Rhyacophila fasciata viteceki Valladolid Kučinić (ssp. nov.) from Bosnia and Herzegovina (Trichoptera: Rhyacophilidae).

We present the description of two new subspecies of the Rhyacophila fasciata Group: Rhyacophila fasciata delici Kučinić Valladolid (ssp. nov.), broadly distributed in Croatia and present also in Bosnia and Herzegovina, and R.

The Rhyacophila fasciata Group in Greece: Rhyacophila kykladica Malicky amp; Sipahiler 1993 (stat. prom.) (Trichoptera: Rhyacophilidae). Morphological description, genetic and ecological features.

The morphology of the different stages (larva, pupa, male, and female) of Rhyacophila fasciata kykladica Malicky Sipahiler 1993 was examined as a basis for the description of the taxon. Morphological data were supplemented by molecular analysis of mitochondrial cytochrome C oxidase subunit I (mtCOI), which were compared with samples of the nominate subspecies Rhyacophila fasciata fasciata Hagen 1859, as well as with other new species in this group, Rhyacophila denticulata McLachlan 1879 and Rhyacophila sociata Navás 1916. Our results revealed genetic differences between all the taxa; therefore, a change of taxonomic status of R.

Assessment of the risks of N-loss to groundwater from data on N-balance surplus in Spanish crops: An empirical basis to identify Nitrate Vulnerable Zones.

The aim of this research was to conduct an empirical assessment of the risks of N-loss to groundwater associated with land use (LU), based on annual data on the net N-balance surplus in Spanish crops. These data were used to generate a detailed risk rating system reflecting the potential risks of N-loss from agriculture. The new LU ratings were used to assess the specific vulnerability of groundwater to nitrate pollution, by using the LU-IV procedure (Arauzo 2017).

The Rhyacophila fasciata Group in Western Europe: Confirmation of Rhyacophila denticulata McLachlan 1879 (stat. prom.) and Rhyacophila sociata Navás 1916 (stat. res.), based on morphological and molecular genetic evidence (Trichoptera: Rhyacophilidae).

In order to check the presence and distribution of Rhyacophila fasciata fasciata Hagen 1859 (species described from Austria) and R. fasciata denticulata McLachlan 1879 in the Iberian Peninsula, we studied the morphology of Spanish, French, and Austrian specimens, together with their mitochondrial cytochrome c oxidase (mtCOI). We observed that the individuals considered to date as R.

Growth and reproductive biology of loaches Cobitis sp. in Lake Lucień, Poland.

Five age-classes and their corresponding average body lengths of loaches from Lake Lucień were determined by the Bhattacharya method. The maximum observed length was 112 mm for females and 91 mm for males. The von Bertalanffy equation defining growth pattern was L(T) = 116 (1-exp (-0.

Feeding activity of spined loach Cobitis sp. in Lake Lucień, Poland.

The feeding activity of spined loach Cobitis sp. was studied in post-glacial ribbon Lake Lucień, Central Poland, during three 24 hrs periods in May, August and September. Samples were taken every 4 hrs and 8 to 18 (mode 10) specimens were caught on each sampling occasion.

Feeding ecology of Cobitis paludica and Cobitis calderoni in Central Spain.

In total, 253 specimens of Cobitis paludica and 163 Cobitis calderoni were collected in the Lozoya River (Madrid, Spain) in April, May, July, September and December 1990. Both species showed high percentages of empty guts in all months. C.