A vaccine combining ORF132 and ORF25 expressed by Saccharomyces cerevisiae induces protective immunity in Carassius auratus gibelio against CyHV-2.

CyHV-2 is the pathogen of herpesvirus hematopoietic necrosis (HVHN), resulting in significant economic losses in crucian carp. Although multiple oral vaccines have been developed to prevent CyHV-2, they have not achieved ideal protective effects. To improve the protective effect of oral vaccine, a combination vaccine was conducted by mixing recombinant Saccharomyces cerevisiae displaying ORF132 or ORF25 on the cell surface in a 1:1 ratio.

Immersion immunization with recombinant Saccharomyces cerevisiae displaying ORF25 induced protective immunity against cyprinid herpesvirus 2.

Displaying antigens on yeast surface as an oral vaccine has been widely explored, while its potential as an immersion vaccine has not been evaluated. Here, an immersion vaccine was prepared by displaying ORF25 of Cyprinid herpesvirus 2 (CyHV-2) on the surface of Saccharomyces cerevisiae. Carassius auratus gibelio was immersion immunized by 2 × 10 CFU/mL yeast for 2 h, and reinforce the immunity using the same method 14 days after the first immunization.

Pillar[5]arene-segregated ion pairs for enhanced cycloaddition of epoxides with CO.

A supramolecular approach using a polyviologen-pillar[5]arene complex as segregated ion pairs was shown to be highly efficient for the conversion of CO with epoxides into cyclic carbonates without the need for metals or solvents. The enhanced catalytic performance was achieved by cooperative ion pair segregation and CO fixation.

High-Performance Single-Crystal Lithium-Rich Layered Oxides Cathode Materials via NaWO-Assisted Sintering.

Single-crystal lithium-rich layered oxides (LLOs) with excellent mechanical properties can enhance their crystal structure stability. However, the conventional methods for preparing single-crystal LLOs, require large amounts of molten salt additives, involve complicated washing steps, and increase the difficulty of large-scale production. In this study, a sodium tungstate (NaWO)-assisted sintering method is proposed to fabricate high-performance single-crystal LLOs cathode materials without large amounts of additives and additional washing steps.

Anion-Cation Dual-Ion Multisite Doping Stabilizes the Crystal Structure of Li-Rich Layered Oxides.

Li-rich layered oxide (LLOs) cathode materials are gaining increasing attention as lithium-ion batteries (LIBs) pursue greater energy density. However, LLOs still suffer from severe capacity fading and voltage decay due to their unstable crystal structure. Hence, the anion-cation dual-ion multisite doping strategy based on Mg and S atoms is used to stabilize the crystal structures of LLOs.

LiNO-Assisted Succinonitrile-Based Solid-State Electrolyte for Long Cycle Life toward a Li-Metal Anode via an In Situ Thermal Polymerization Method.

Succinonitrile (SN)-based electrolytes have a great potential for the practical application of all-solid-state lithium-metal batteries (ASSLMBs) due to their high room-temperature ionic conductivity, broad electrochemical window, and favorable thermal stability. Nevertheless, the poor mechanical strength and low stability toward Li metal hinder the further application of SN-based electrolytes to ASSLMBs. In this work, the LiNO-assisted SN-based electrolytes are synthesized via an in situ thermal polymerization method.

Oral Vaccination of Recombinant Expressing ORF132 Induces Protective Immunity against Cyprinid Herpesvirus-2.

Cyprinid herpesvirus 2 (CyHV-2) is the etiological agent of herpesviral hematopoietic necrosis (HVHN) disease, which causes serious economic losses in the crucian carp culture industry. In this study, by displaying ORF132 on the surface of cells (named EBY100/pYD1-ORF132), we evaluated the protective efficacy of oral administration against CyHV-2 infection. Intense innate and adaptive immune responses were evoked in both mucosal and systemic tissues after oral vaccination with EBY100/pYD1-ORF132.

Supramolecular Room Temperature Phosphorescent Materials Based on Cucurbit[8]uril for Dual Detection of Dodine.

In recent years, host-guest interactions of macrocycles have attracted much attention as an emerging method for enhancing the intersystem crossing of pure organic room-temperature phosphorescence. In this work, we utilize cucurbit[8]uril (Q[8]) to specifically recognize synthetic bromophenyl pyridine derivatives (BPCOOH) to construct a highly stable charge-transfer dimer, where the bromophenyl pyridine moiety of BPCOOH is encapsulated by Q[8] in a 1:2 host/guest ratio. The assemblies exhibit specific recognition and detection properties for dodine on both fluorescence and phosphorescence spectra.

Cucurbit[10]uril-mediated Supramolecular Assembly for Optically Tunable Dimers and Near White-light Emissive Materials.

Cucurbit[10]uril (Q[10]), the cucurbit[n]uril with a large cavity, exhibits several new features in the development of the host-guest complex. Thus, based on Q[10] and π-conjugated molecule, oligo(p-phenylenevinylene) derivative (OPVCOOH), the host-guest complexes with three different interaction ratios of 1 : 2, 2 : 2, and 3 : 2 assemblies (Q[10]: guest) were fabricated. Depending on the host/guest ratio, the emission color of these complexes ranged from blue to yellow-green.

Analysis of the Effect of Nine Consecutive Years' Intensive Management and Number of Times Achieving the Target Control on Endpoint Events in T2DM Patients in Sanlitun Community Health Service Center in Beijing.

Objective: To investigate the effect of intensive management and achieving the target control more than 3 times on endpoint events during 9 consecutive years' annual assessment in type 2 diabetes (T2DM) patients in the Sanlitun Community Health Service Center in Beijing, including blood glucose, blood pressure, lipids profiles, and the joint target control.

Methods: In Beijing Community Diabetes Study (BCDS), 224 patients with T2DM from the Sanlitun Community Health Service Center were enrolled in 2008. All patients were randomly assigned to the intensive management group ( = 113) and the standard management group ( = 113) and the standard management group (.

Lysimachia christinae Hance regresses preestablished cholesterol gallstone in mice.

Ethnopharmacological Relevance: Whole herb of Lysimachia christinae has long been used as a remedy for cholelithiasis extensively in China. This study was aimed to validate the effect of L. christinae on eliminating preestablished cholesterol gallstone (CGS) in model animals.

Electrochemical behavior and determination of rutin on a pyridinium-based ionic liquid modified carbon paste electrode.

In this paper the electrochemical behavior of rutin on a pyridinium-typed ionic liquid modified carbon paste electrode (IL-CPE) was investigated and further used for rutin sample determination. The IL-CPE showed strong electrocatalytic effects to the oxidation of rutin. In phosphate buffer solution (PBS, pH 2.

Electrocatalytic oxidation of dopamine at an ionic liquid modified carbon paste electrode and its analytical application.

A room-temperature ionic liquid N-butylpyridinium hexafluorophosphate was used as a binder to construct an ionic liquid modified carbon paste electrode, which was characterized by scanning electron microscopy and electrochemical impedance spectroscopy. The ionic liquid carbon paste electrode (IL-CPE) showed enhanced electrochemical response and strong analytical activity towards the electrochemical oxidation of dopamine (DA). A pair of well-defined quasireversible redox peaks of DA appeared, with the redox peaks located at 215 mV (E (pa)) and 151 mV (E (pc)) (vs.