Reconstruction and analysis of the industrial strain Bacillus megaterium WSH002 genome-scale in silico metabolic model.

A genome-scale metabolic model of Bacillus megaterium WSH002, an industrial bacterium widely used in the vitamin C industry, was reconstructed on the basis of the genome annotation and data from the literature and biochemical databases. It comprises 1112 reactions, 993 metabolites, and 1055 genes, including 43 new annotated genes. This model was able to predict qualitatively and quantitatively the growth of B.

[Reconstruction and analysis of Bacillus megaterium metabolic model based on literature study].

Objective: The aim of this study is to reconstruct the metabolic model of Bacillus megaterium by literature study, which would be used to elucidate physiological properties in detail and refine physiological functions.

Methods: We built a literature database by searching B. megaterium related literatures from Web of Science, PubMed, United States Patent and Trademark Office (USPTO) Patent Databases, Derwent Innovations Index and China National Knowledge Infrastructure (CNKI).

Reconstruction and analysis of a genome-scale metabolic model of the vitamin C producing industrial strain Ketogulonicigenium vulgare WSH-001.

Ketogulonicigenium vulgare WSH001 is an industrial strain commonly used in the vitamin C producing industry. In order to acquire a comprehensive understanding of its physiological characteristics, a genome-scale metabolic model of K. vulgare WSH001, iWZ663, including 830 reactions, 649 metabolites, and 663 genes, was reconstructed by genome annotation and literature mining.