Electrophysiological and behavioral responses of Ips duplicatus to aggregation pheromone in Inner Mongolia, China: amitinol as a potential pheromone component.

Volatiles from Ips duplicatus male hindgut extracts and aeration samples of spruce logs colonized by the virgin males were analyzed by coupled gas chromatography-mass spectrometry (GC-MS), coupled gas chromatography-electroantennographic detection (GC-EAD), and field trapping bioassays in Inner Mongolia, China. GC-MS analyses showed that males from nuptial chambers (for reproduction) produced hindgut volatile profiles similar to those of maturation feeding males from the nonreproductive galleries. The known aggregation pheromone components, ipsdienol (Id) and E-myrcenol (EM) are the major constituents of this blend, followed by several minor bark beetle-related compounds: 2-methyl-3-buten-2-ol, ipsenol, cis-verbenol, verbenone, 2-phenylethanol, myrtenol, and trans-myrtanol.