The expression of CD86 in CD3CD56 NKT cells is associated with the occurrence and prognosis of sepsis-associated encephalopathy in sepsis patients: a prospective observational cohort study.

The role of CD3CD56 natural killer T (NKT) cells and its co-signaling molecules in patients with sepsis-associated encephalopathy (SAE) is unknown. In this prospective observational cohort study, we initially recruited 260 septic patients and eventually analyzed 90 patients, of whom 57 were in the SAE group and 37 were in the non-SAE group. Compared to the non-SAE group, 28-day mortality was significantly increased in the SAE group (33.

Paclitaxel induces apoptosis through the TAK1-JNK activation pathway.

Paclitaxel (PTX) has previously been used to treat tumours of various tissue origins, such as lung, breast, ovarian, prostate cancers and leukemia. PTX-induced apoptosis is associated with p38 mitogen-activated protein kinase (p38 MAPK), extracellular signal-regulated kinase (ERK), nuclear factor-kappa B (NF-κB) and c-Jun N-terminal kinase or stress-activated protein kinase (JNK/ SAPK) pathways. Transforming growth factor-beta-activated kinase 1 (TAK1) and TAK1-binding protein 1 (TAB1) play an important role in cell apoptosis through the p38, ERK, NF-κB and JNK signal transduction pathways.

High Expression of CCDC34 Is Associated with Poor Survival in Cervical Cancer Patients.

BACKGROUND The present study explored the expression of coiled-coil domain-containing 34 (CCDC34) in cervical cancer (CC) and its prognostic value. MATERIAL AND METHODS GEPIA and Oncomine cancer databases were mined to predict the CCDC34 differential expression level between a CC group and a normal group. Immunohistochemistry was performed to examine the CCDC34 expression in 67 CC and corresponding adjacent tissues.

[Detection of NK and NKT cells in peripheral blood of patients with cGVHD and its significance].

The aim of this study was to investigate the correlation of NK and NKT cells in peripheral blood of patients undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT) with chronic graft-versus-host disease (cGVHD). 64 patients undergoing allo-HSCT in Guangdong Provincial People Hospital were studied retrospectively. Among 64 cases, 21 cases were did not develop with cGVHD, 43 cases (mild 15, moderate 18, severe 10) were recorded with cGVHD.

[Effect of peripheral blood mononuclear cells from patients with obstructive sleep apnea-hypopnea syndrome on apoptosis of vascular endothelial cells].

Objective: To investigate the mechanism of vascular endothelial cell injury in patients with obstructive sleep apnea-hypopnea syndrome (OSAHS).

Methods: Peripheral blood samples were collected early in the morning from 20 normal persons, 21 patients with mild OSAHS, 24 patients with moderate OSAHS, and 20 patients with severe OSAHS according to the results of polysomnography (PSG). Mononuclear cells (MNCs) were isolated and co-cultured with human umbilical vein endothelial cells of the strain ECV304 for 48 h.

[Immunophenotypic features of acute myeloid leukemia with AML-1/ETO fusion gene].

AML-1/ETO fusion gene is the frequent genetic lesion described in FBA M(2) type acute myeloid leukemia (AML-M(2)) and is associated with a favourable prognosis. In spite of its potential clinical relevance, this subtype leukemia usually would be undetected with conventional cytology procedures, and easily confused with acute promyelocyte leukemia (APL) in morphology. In order to investigate the immunophenotypic characteristics of bone marrow cells in AML-M(2) patients with AML-ETO gene rearrangement classified by FAB, immunophenotype of bone marrow cells in 17 AML-M(2) patients with AML-1/ETO(+) confirmed by fluorescence in situ hybridization was analyzed by using flow cytometry as compared with immunophenotype in 34 APL patients with AML-1/ETO(-).

[Cytokine expression of CD4+ and CD8+ T cells induced by different stimuli under different culture conditions].

Aim: To investigate the effect of different stimuli and different culture conditions on the cytokine expression of CD4+ and CD8+ T cells.

Methods: PBMCs were isolated from normal human peripheral blood and cultured with three kinds of stimuli (PHA, anti-CD3 and anti-CD28 mAbs, PMA and ionomycin) under four different culture conditions (room temperature, 37 degrees C water bath, 37 degrees C incubator, 37 degrees C and 50 mL/L CO2 incubator) for 4.5 ~ 5 h and intracellular cytokines (IL-2, IFN-gamma and TNF-alpha) in T cells were assessed by flow cytometry (FCM).