Publications by authors named "Mao Nakagawa"
Outer membrane vesicles (OMVs) are produced by Gram-negative bacteria and deliver microbial molecules to distant target cells in a host. OMVs secreted by probiotic probiotic strain Escherichia coli Nissle 1917 (EcN) have been reported to induce an immune response. In this study, we aimed to increase the OMV production of EcN.
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- - The study focuses on the production of outer membrane vesicles (OMVs) by certain mutant cells, specifically ΔΔ, which exhibit increased OMV generation due to genetic modifications affecting membrane structure and phospholipid levels.
- - Using advanced imaging techniques, researchers found that plasmolysis, a process where the cell membrane pulls away from the cell wall, occurred at the ends of these mutant cells, leading to OMV formation.
- - Notably, the ΔΔ cells showed a significant increase in the secretion of a recombinant protein and had larger holes in their peptidoglycan layer, suggesting that materials from the cytoplasm are being pushed into the outer-inner membrane vesicles (OIMVs) and released into the environment