Amino acid deletions at positions 893 and 894 of cytotoxin-associated gene A protein affect gastric epithelial cell interactions.

Background: () persistently colonizes the human gastric mucosa in more than 50% of the global population, leading to various gastroduodenal diseases ranging from chronic gastritis to gastric carcinoma. Cytotoxin-associated gene A (CagA) protein, an important oncoprotein, has highly polymorphic Glu-Pro-Ile-Tyr-Ala segments at the carboxyl terminus, which play crucial roles in pathogenesis. Our previous study revealed a significant association between amino acid deletions at positions 893 and 894 and gastric cancer.

Establishment and Modification of Ninety-seven Pneumococcal Serotyping Assays Based on Quantitative Real-time Polymerase Chain Reaction.

Objective: To establish and modify quantitative real-time polymerase chain reaction (qPCR)-based serotyping assays to distinguish 97 pneumococcal serotypes.

Methods: A database of capsular polysaccharide ( ) loci sequences was generated, covering 97 pneumococcal serotypes. Bioinformatics analyses were performed to identify the loci structure and target genes related to different pneumococcal serotypes with specific SNPs.

A Campylobacteriosis Outbreak Caused by One Asymptomatic Food Handler Carrier.

In August 2021, three students with diarrhea from the same school visited a local hospital in the S district of Beijing. An epidemic investigation showed that there were more students with diarrhea in the same school and they had one meal together. was isolated from both patients with diarrhea and asymptomatic food handlers; however, the latter also carried .

Genetic Characteristics of Lipooligosaccharide and Capsular Polysaccharide of from Different Sources in China.

Objective: To determine the distribution of two important virulence factors [lipooligosaccharide (LOS) and capsular polysaccharide (CPS)] in ( ) isolated from different sources in China and to develop a rapid screening method for Guillain-Barré syndrome (GBS)-associated strains.

Methods: Whole-genome sequencing was carried out for 494 strains. The OrthoMCL software was used to define the LOS/CPS gene clusters.

Comparison of the Pathogenicity of Isolates of Hyperinvasive Sequence Type 7 Belonging to Serogroups A, B, C, and X.

Objective: To compare the pathogenicity of isolates of sequence type 7 (ST-7) ( ) belonging to four different serogroups (A, B, C, and X).

Methods: Four ST-7 isolates serogrouped as A, B, C, and X and characterized by different capsule structures, were examined for their adhesion and invasion properties, and their ability to induce cytokine release and apoptosis in the host cell (the A549 cell line).

Results: Among the four ST-7 isolates, the serogroup A isolate possessed the strongest adhesion and invasion ability.

Genetic and Antibiotic Resistance Characteristics of Campylobacter jejuni Isolated from Diarrheal Patients, Poultry and Cattle in Shenzhen.

Objective: To investigate genetic and antibiotic resistance characteristics of Campylobacter jejuni (C. jejuni) isolated from Shenzhen.

Methods: Multilocs sequence typing and agar dilution methods were used to define the genotype and antibiotic resistance of C.

Effect of GG supernatant on serotonin transporter expression in rats with post-infectious irritable bowel syndrome.

Aim: To evaluate the effect of GG supernatant (LGG-s) on the expression of serotonin transporter (SERT) in rats with post-infectious irritable bowel syndrome (PI-IBS).

Methods: 81-176 (10 CFU/mL) was used to induce intestinal infection to develop a PI-IBS model. After evaluation of the post-infectious phase by biochemical tests, DNA agarose gel electrophoresis, abdominal withdrawal reflex (AWR) test, and the intestinal motility test, four PI-IBS groups received different concentrations of LGG-s for 4 wk.

Development and application of a real-time polymerase chain reaction method for Campylobacter jejuni detection.

Aim: To develop a real-time polymerase chain reaction (PCR) method to detect and quantify Campylobacter jejuni (C. jejuni) from stool specimens.

Methods: Primers and a probe for real-time PCR were designed based on the specific DNA sequence of the hipO gene in C.

In vitro protein expression profile of Campylobacter jejuni strain NCTC11168 by two-dimensional gel electrophoresis and mass spectrometry.

Objective: To investigate the protein expression profiles of the major food-borne pathogen Campylobacter jejuni NCTC11168.

Methods: Membrane and soluble cellular proteins were extracted from the genome-sequenced C. jejuni strain NCTC11168.

[Polymorphism of Helicobacter pylori vacA, isolated in China].

Objective: To understand the polymorphism of Helicobacter pylori (H. pylori) vacA alleles in China.

Methods: A total of 119 H.

Comparative proteomic analysis of Campylobacter jejuni cultured at 37C and 42C.

Campylobacter jejuni has the potential to thrive at 37C (e.g., in the human intestinal tract) and 42C (e.

Comparative proteomic analysis of passaged Helicobacter pylori.

In order to identify the proteins associated with Helicobacter pylori colonization in mice, we used 2-dimensional gel electrophoresis (2-DE) to analyze the membrane- and soluble-cellular proteins extracted from H. pylori strain 26695 and the mouse-passaged homolog 88-3887. We defined 2- and 3-fold changes in protein expression as the threshold values for differential expression in the membrane-protein and whole-cell-protein fractions, respectively.

Genetic analysis of group A streptococcus isolates recovered during acute glomerulonephritis outbreaks in Guizhou Province of China.

In this study, 68 group A streptococcus (GAS) isolates associated with two outbreaks of acute glomerulonephritis (AGN) in China were analyzed by emm typing. A total of 11 different emm types were identified. Analysis of emm type distribution suggested that AGN outbreaks in two counties were caused by emm60.

[Multi-PCR identification and virulence genes detection of Campylobacter jejuni isolated from China].

Objective: This study was to simultaneously identify Campylobacter jejuni and Campylobacter coli isolates in China by Multi-PCR assay and to study the prevalence of six virulence and toxin genes on them.

Methods: A multi-PCR method with three sets of primers specifically designed for application of a 16S rRNA as a universal control, mapA, ceuE based on the specific sequence of C. jejuni and C.

Cross-reactivity of anti-H pylori antibodies with membrane antigens of human erythrocytes.

Aim: To investigate whether anti-H pylori antibodies have cross-reaction with antigens of erythrocyte membrane.

Methods: Blood samples were collected from 14 volunteers (8 positive and 6 negative for H pylori detected by (13)C-urea breath test) of the general population. Erythrocyte membrane proteins of the subjects were examined by Western blot using anti-H pylori serum.

Sequence organization and insertion specificity of the novel chimeric ISHp609 transposable element of Helicobacter pylori.

Here we describe ISHp609 of Helicobacter pylori, a new member of the IS605 mobile element family that is novel and contains two genes whose functions are unknown, jhp960 and jhp961, in addition to homologs of two other H. pylori insertion sequence (IS) element genes, orfA, which encodes a putative serine recombinase-transposase, and orfB, whose homologs in other species are also often annotated as genes that encode transposases. The complete four-gene element was found in 10 to 40% of strains obtained from Africa, India, Europe, and the Americas but in only 1% of East Asian strains.