Recombinant Antibody-Producing Stable CHOK1 Pool Stability Study.

Mammalian cell line stability is an important consideration when establishing a biologics manufacturing process in the biopharmaceutical and diagnostics (IVD) industries. Traditional Chinese hamster ovary (CHO) cell line development methods use a random integration approach that requires transfection, selection, optional amplification, screenings, and single-cell cloning to select clones with acceptable productivity, product quality, and genetic stability. Site-specific integration reduces these disadvantages, and new technologies have been developed to mitigate risks associated with genetic instability.

Iodine Nutrition, Thyroid-stimulating Hormone, and Related Factors of Postpartum Women from three Different Areas in China: A Cross-sectional Survey.

Objective: Studies on the relationship between iodine, vitamin A (VA), and vitamin D (VD) and thyroid function are limited. This study aimed to analyze iodine and thyroid-stimulating hormone (TSH) status and their possible relationships with VA, VD, and other factors in postpartum women.

Methods: A total of 1,311 mothers (896 lactating and 415 non-lactating) from Hebei, Zhejiang, and Guangxi provinces were included in this study.

CLIC4 Regulates Endothelial Barrier Control by Mediating PAR1 Signaling via RhoA.

Background: Endothelial CLICs (chloride intracellular channel proteins) CLIC1 and CLIC4 are required for the GPCRs (G-protein-coupled receptors) S1PR1 (sphingosine-1-phosphate receptor 1) and S1PR3 to activate the small GTPases Rac1 (Ras-related C3 botulinum toxin substrate 1) and RhoA (Ras homolog family member A). To determine whether CLIC1 and CLIC4 function in additional endothelial GPCR pathways, we evaluated CLIC function in thrombin signaling via the thrombin-regulated PAR1 (protease-activated receptor 1) and downstream effector RhoA.

Methods: We assessed the ability of CLIC1 and CLIC4 to relocalize to cell membranes in response to thrombin in human umbilical vein endothelial cells (HUVEC).

CLIC1 and CLIC4 mediate endothelial S1P receptor signaling to facilitate Rac1 and RhoA activity and function.

Chloride intracellular channels 1 (CLIC1) and 4 (CLIC4) are expressed in endothelial cells and regulate angiogenic behaviors in vitro, and the expression of is important for vascular development and function in mice. Here, we found that CLIC1 and CLIC4 in endothelial cells regulate critical G protein-coupled receptor (GPCR) pathways associated with vascular development and disease. In cultured endothelial cells, we found that CLIC1 and CLIC4 transiently translocated to the plasma membrane in response to sphingosine 1-phosphate (S1P).

Basal Energy Expenditure of Chinese Healthy Adults: Comparison of Measured and Predicted Values.

Objective: This study aimed to measure the basal energy expenditure (BEE) of Chinese healthy adults and establish an accurate predictive equation for this population.

Methods: In total, 470 Chinese healthy adults had their BEE measured using the Cosmed K4b portable metabolic system. Multiple linear regression analysis was applied to develop new optimal equations for predicting BEE.

Sphingosine-1-Phosphate Receptor 1 Activity Promotes Tumor Growth by Amplifying VEGF-VEGFR2 Angiogenic Signaling.

The vascular endothelial growth factor-A (VEGF-A)-VEGFR2 pathway drives tumor vascularization by activating proangiogenic signaling in endothelial cells (ECs). Here, we show that EC-sphingosine-1-phosphate receptor 1 (S1PR1) amplifies VEGFR2-mediated angiogenic signaling to enhance tumor growth. We show that cancer cells induce S1PR1 activity in ECs, and thereby, conditional deletion of S1PR1 in ECs (EC-S1pr1 mice) impairs tumor vascularization and growth.

[Sustainability science revisited: Recent advances and new opportunities].

During the past decade, sustainability science has rapidly developed into a globally well-recognized and important new science of the 21st century. However, sustainability science has not received much attention from scientists and practitioners in China where sustainable development and ecological civilization have been prominent themes. To promote the development of sustainability science in China, Wu, et al.

Omega-3 fatty acids suppress Fusobacterium nucleatum-induced placental inflammation originating from maternal endothelial cells.

Fusobacterium nucleatum is an oral anaerobe prevalent in intrauterine infection associated with a wide spectrum of adverse pregnancy outcomes. We demonstrate here that F. nucleatum triggers placental inflammation through maternal, rather than paternal, TLR4-mediated signaling.

[Dynamics of habitat suitability for waterfowls from 1990 to 2015 in the ecological function zone of Sanjiang Plain, Northeast China].

As a wetland of international importance, the ecological function zone of the Sanjiang Plain (EFZSP) plays an important role in maintaining waterfowl habitat. With the increasing disturbance of human beings, land use and climate changes, habitat suitability for waterfowls (HSW) has drawn greater attention of ecologists. In this study, Landsat TM/ETM+/OLI and HJ-1B images in 1990, 2000, 2010 and 2015 were used to obtain land cover classification by object-based image analysis, and the entropy and analytic hierarchy process methods were adopted to determine the factors and their weights, the factors selected in this study included water condition (lake and river density), disturbance factor (residence and road density), shelter condition (land cover type and slope), and food abundance (NDVI).

Dynamics of Rodent and Rodent-borne Disease during Construction of the Three Gorges Reservoir from 1997 to 2012.

Objective: To investigate the impact of impoundment and active public health interventions on rodent populations and rodent-borne diseases in the Three Gorges reservoir region from 1997 to 2012.

Methods: Surveillance data from 1997 to 2012 were extracted from the Public Health Surveillance System of The Three Gorges established in 1997. Temporal changes in the incidences of hemorrhagic fever with renal syndrome (HFRS) and leptospirosis, rodent density, pathogen-carrying rates, and their correlations were analyzed.

[Impact of maternal genetic effect on genetic parameter estimation of production traits for Qinghai fine-wool sheep].

The maternal genetic effects on estimating genetic parameters for growth traits and wool traits of Qinghai fine-wool sheep were investigated.The genetic parameters for production traits of Qinghai fine-wool sheep were estimated by average information restricted maximum likelihood (AIREML) with different animal models, and the differences between different animal models were tested by likelihood ratio test. Fixed effects, direct genetic effects, and residual effects were included all models; and random effects were individual permanence environmental effects, maternal genetic effects, and maternal permanence environmental effects.

Three days of static stretching within a warm-up does not affect repeated-sprint ability in youth soccer players.

This study aimed to examine the repeated-sprint ability (RSA) in soccer players after 3 days of static stretching. Twenty soccer players (age: 16.8 ± 0.

Temporal characteristics of foot movement in Tai Chi exercise.

The concept of proper foot movement is always emphasized in the practice of Tai Chi. Sixteen experienced Tai Chi practitioners participated in this study. Each subject practiced the whole set of 42-form Tai Chi movements and the performance was video-recorded and analyzed.

Higher plantar pressure on the medial side in four soccer-related movements.

Objective: To measure the plantar pressure in four soccer-related movements in 15 male soccer players (mean (SD) age 20.9 (1.3) years, height 173 (4) cm, weight 61.

Difference in plantar pressure between the preferred and non-preferred feet in four soccer-related movements.

Objective And Participants: The present study measured the difference in plantar pressure between the preferred and non-preferred foot in four soccer-related movements in 15 male university soccer players (mean (SD) age 20.9 (1.3) years, mean (SD) height 173 (4) cm and mean (SD) weight 61.

Plantar pressure distribution during Tai Chi exercise.

Objectives: To describe and quantify the plantar pressure distribution characteristics during Tai Chi exercise and to explain the beneficial effect of Tai Chi on balance control and muscle strength when compared with normal walking.

Design: Description and within-subject design.

Setting: A biomechanics laboratory.

Characteristics of foot movement in Tai Chi exercise.

Background And Purpose: The concept of proper foot movement is always emphasized in practicing Tai Chi (TC). The purposes of this study were to describe the foot movement characteristics of TC and to compare duration of single- and double-limb support time during TC and during walking.

Subjects: Sixteen experienced TC practitioners participated in the study.