Whole genome analysis of multidrug-resistant isolate collected from drinking water in Armenia revealed the plasmid-borne mediated colistin resistance.

Unlabelled: The rate of polymyxin-resistant , as well as human and animal infections caused by them, is increasing worldwide, posing a high epidemiological threat since colistin represents a last-resort antibiotic to treat complicated infections. The study of environmental niches, in particular, aquatic ecosystems in terms of genome analysis of inhabiting antimicrobial-resistant (AMR) microorganisms as reservoirs of acquired resistance determinants (AMR genes), represents a specific concern from a One Health approach. Here, we present a phenotypic AMR analysis and molecular characterization of isolate found in municipal drinking water after an accident in the water supply system of a residential building in Armenia in 2021.

Plasmid Composition, Antimicrobial Resistance and Virulence Genes Profiles of Ciprofloxacin- and Third-Generation Cephalosporin-Resistant Foodborne Isolates from Russia.

is an important foodborne pathogen worldwide. Ciprofloxacin and extended-spectrum cephalosporins are the common first-line antimicrobial drugs for the treatment of salmonellosis, antimicrobial resistance genes for which are mostly transferred via plasmids. The goal of this work was to perform genomic analysis of plasmids from foodborne isolates obtained in Russia based on whole-genome sequencing.

Whole-Genome Analysis of Isolates from Ready-to-Eat Food in Russia.

This study provides a thorough investigation of a diverse set of antimicrobial resistant (AMR) isolates collected from a broad range of ready-to-eat (RTE) food in various geographic regions of Russia ranging from Pskov to Kamchatka. Thirty-five isolates were characterized using the whole genome sequencing (WGS) analysis in terms of clonal structure, the presence of resistance and virulence determinants, as well as plasmid replicon sequences and CRISPR/Cas systems. To the best of our knowledge, this is the first WGS-based surveillance of Russian RTE food-associated isolates.

Comparative Whole-Genome Analysis of Russian Foodborne Multidrug-Resistant Infantis Isolates.

Non-typhoidal infections remain a significant public health problem worldwide. In this study, we present the first detailed genomic analysis report based on short-read (Illumina) whole-genome sequencing (WGS) of 45 multidrug-resistant (MDR) subsp. serotype Infantis isolates from poultry and meat product samples obtained in Russia during 2018-2020, and long-read (MinION) WGS of five more representative isolates.

[The new possibilities in serologic diagnostic of Ixodes mite-borne borreliosis using ImmunoChip].

The new kit of reagents in format of the immunochip "ImmunoChip Borreliosis" for multiplex serologic analysis of mite-borne borreliosis demonstrated high diagnostic sensitivity and specificity. The percentage of detection of specific immunoglobulins was higher in "ImmunoChip Borreliosis" as compared with screening results in immune enzyme analysis. The high correlation between results of testing in immunochip and data of immune blotting is demonstrated to.

[Assessing the conformity of medical devices for in vitro diagnosis: international and domestic experience].

The conformity of medical articles was assessed for in vitro diagnosis is the most importance procedure for their entering the market. This paper describes the basic elements of assessment of the conformity of these articles, analyzes worldwide experience, shows differences in the Russian regulatory system in the circulation of medical articles for in vitro diagnosis, and gives recommendations for its improvement.

[Design of an immunochip for separate detection of hepatitis C virus antibodies].

A test kit as an immunochip designed for the diagnosis of hepatic C virus (HCV) has a high sensitivity and specificity. Recombinant HCV antigens were separately immobilized on the activated slides together with internal controls. Serum test results were red by ScanArray Express.

[ELISA and PCR test systems used to detect Crimean-Congo hemorrhagic fever virus].

Studying the sensitivity and specificity of enzyme-linked immunosorbent assay (ELISA) for the indication of Crimean-Congo hemorrhagic fever (CCHF) virus antigens and those of reverse transcription and polymerase chain reaction (RT-PCR) for the detection of CCHF virus RNA, and those of a intercerebral infection method in newborn albino mice systems for the determination of viral infectious activity established that the sensitivity of ELISA was 1-2 orders of magnitude less than that of RP-PCR. The latter proved to be better in studying the sera sampled from patients with CCHF. The results of studying the samples of H.

[Preparations for serodiagnosis of diseases due to causative agents of ixodes tick-borne borreliosis (Lyme disease). Communication 3. Comparative study of enzyme immunoassay test-systems for detection of IgM to Borrelia burgdorferi sensu lato].

Three foreign and one Russian ELISA test-systems for detection of IgM to Borrelia burgdorferi sensu lato were comparatively studied with the use of the clinical material in an encoded experiment. In the foreign ELISA test-systems based on the use of native antigens of borrelia, cross reactions with sera from patients with syphilis, Epstein-Barr infection, cytomegalovirus infection and systemic lupus erythematosus were detected. The Russian recombinant ELISA test-system Borreliosis-ELISA-IgM showed high sensitivity and specificity.

[Preparations for serodiagnosis of diseases due to causative agents of ixode tick-borne Borreliosis (Lyme disease). Communication 2. Comparative study of recombinant enzyme immunoassay test-systems (rELISA) for serological diagnosis of ixode tick-borne Borreliosis].

One foreign and two Russian recombinant enzyme immunoassay test-systems were comparatively investigated under conditions of an encoded experiment. Sensitivity in the experiment with the Russian test-systems Borreliosis-ELISA-IgG and Lyme Best was 63.8 and 68.

[Preparations for serodiagnosis of diseases due to causative agents of ixode tick-borne borreliosis (Lyme disease). Communication 1. Comparative study of the 1st generation enzyme immunoassay test-systems for detection of antibodies to Borrelia burkdorferi sensu lato].

Four foreign and one Russian 1st generation test-systems for detecting class G antibodies or summary antibodies to Borrelia burkdorferi sensu lato were comparitively investigated with the use of the clinical material under conditions of an encoded experiment. Cross reactions with sera from patients with syphilis, Epstein-Barr infection, cytomegalovirus infection and systemic lupus erythematosus were observed. The best specificity and sensitivity parameters were provided by the Enzygnost Borreliosis test-system.

[Evaluation of the first domestic immunoenzyme recombinant test-system "Khlami-IGA-DS-Tr" for detection of class A antibodies to Chlamydia trachomatis].

A test system for detecting species-specific class A antibodies to Chlamydia trachomatis, making use of recombinant chlamydial antigen, is developed in Russia for the first time. Its efficiency was demonstrated in blind clinical trials. The sensitivity, specificity, activity, and use of Chlamy-IgA-DC-Tr test system are not inferior to foreign analogs.

[Serodiagnosis of chlamydiosis: "Chlamy-IgG-DS-Tr"-- the first domestic immunoenzyme recombinant test-system for determination of anti-Chlamydia trachomatis class G antibodies].

The first Native test-system for determination of class G antibodies to Chlamydia trachomatis with chlamydia recombinant antigen was elaborated Test-system efficacy was demonstrated in clinical trials. The sensibility, specific activity and suitability of the "Chlamy-IgG-DS-Tr" were the same as for import analogous.

[Burkholderia mallei and Burkholderia pseudomallei. Study of immuno- and pathogenesis of glanders and melioidosis. Heterologous vaccines].

Parameters of the infectious activity of B.mallei and B.pseudomallei for animals of various species were determined.

[Sensitivity of Escherichia coli O157:H7, a pathogen of hemorrhagic colitis, to antibacterial drugs].

Antibioticograms of enterohemorrhagic strains of serogroup O157 Escherichia coli isolated in the Russian Federation and Japan were comparatively studied. Strains with multiple drug resistance were detected. The main biochemical characteristics of the isolates were investigated.

[The principles of the therapy of glanders in monkeys].

The effect of pathogenetic therapy in the normalization of homeostasis disturbances in monkeys has been shown under experimental conditions. Data on the possibility of using hemosorption in the treatment of severe forms of glanders are presented. The conclusion on the necessity of using complex treatment for the effective therapy of glanders in humans has been made.

[Homeostatic changes in monkeys in a model of glanders].

Some aspects of homeostasis impairment in monkeys infected with Pseudomonas mallei were investigated. The levels of the hormonal shifts, complement components, beta 2-microglobulin and prostaglandins evident of the infection severity were estimated. The pathomorphological profiles of various forms of malleus in the primates were studied.

[Pasteurella multocida: infections in man].

The literature data on human infection due to a representative of the genus Pasteurella, i.e. P.

[Erysipelothrix rhusiopathiae: plasmids, resistance to antibacterial drugs].

The plasmid profile, virulence and antibacterial drug susceptibility of various strains of E. rhusiopathiae were determined. No correlations between the virulence of the strains, their antibiotic resistance and the plasmid content were detected.

[Conjugative transfer and expression of R plasmids of the genus Pseudomonas in the cells of Pseudomonas mallei C-5].

Conjugative transfer of the incompatibility group plasmids Inc P-2, Inc P-3, Inc P-4 and Inc P-5 as well as the plasmids of Pseudomonas sp. of an unknown incompatibility group to the cells of Pseudomonas mallei C-5 was shown possible for the first time. The inheritance of the transferred plasmids and the expression of the markers of resistance to antibacterial drugs were stable.

[The sensitivity of Pseudomonas mallei strains to antibacterial preparations in in-vitro experiments on a cell culture model].

The cytopathogenic action of P. mallei on macrophages of golden hamsters and transplantable Hep-2 cell culture was studied. It was shown that the culture test systems may be efficient in estimation of the P.

[Various indices of the infectious process in treatment of glanders in monkeys].

The time course of the clinical, biochemical and serological indices was studied during the treatment of malleus in monkeys. The internal organs of the animals infected with Pseudomonas mallei were investigated pathomorphologically. The efficacy of the biseptol/oxalinic acid combination in the therapy of malleus was estimated.

[Resistance of Pseudomonas mallei to tetracyclines: assessment of the feasibility of chemotherapy].

The spectrum of cross resistance in tetracycline resistant strains of Pseudomonas mallei was studied. Possible use of antibacterial drugs in the prevention and treatment of mallel due to such strains was investigated in the experiments on golden hamsters and monkeys. It was shown that the efficacy of the minocycline therapy depended on the level of the strain resistance to tetracycline antibiotics.