Coping with Complications that Occur after Prostate Biopsy for Satisfactory Evaluation of Call Service Using Artificial Intelligence: A Pilot Randomized Controlled Trial.

Purpose: To assess whether an artificial intelligence (AI)-based reassurance-call can inform patients about potential complications and provides reassurance following a prostate biopsy.

Materials And Methods: From October 2022 to December 2023, 42 patients aged 40 to 70 years undergoing their first prostate biopsy were recruited. The 'Reassurance-call' service was utilized to inform and monitor patients for complications.

5-Aminolevulinic acid improves strawberry salt tolerance through a NO-HO signaling circuit regulated by FaWRKY70 and FaWRKY40.

Introduction: 5-Aminolevulinic acid (ALA) is an essential biosynthetic precursor of tetrapyrrole compounds, naturally occurring in all living organisms. It has also been suggested as a new plant growth regulator. Treatment with ALA promotes strawberry Na homeostasis under salt stress.

Evolving Concept of Value in Health Economics and Outcomes Research: Emerging Tools for Innovation and Access to Cell and Gene Therapies for Rare Diseases.

Objective: Recent scientific breakthroughs have propelled the development of disease-modifying and potentially curative cell and gene therapies (CGTs) for rare diseases, including those diseases previously considered untreatable. However, the unique characteristics of CGTs pose challenges for the traditional methods of therapy value determination, reimbursement, and outcome evaluation used by regulatory and assessment agencies for product approval and market access. Notably, CGTs are one-time or short-course treatments, often first-in-class (precluding direct comparisons with effective alternatives), and have health benefits that are largely realized over time.

Enhanced anti-tumor efficacy of berberine-loaded mesoporous polydopamine nanoparticles for synergistic chemotherapy and photothermal therapy.

The development of innovative therapeutic strategies that combine multiple treatment modalities is essential for effective cancer therapy. In this study, we engineered berberine (BER)-loaded mesoporous polydopamine (MPDA) nanoparticles (BER-MPDA) to enhance anti-tumor efficacy through synergistic chemotherapy and photothermal therapy (PTT). The mesoporous structure of MPDA allowed for a high loading capacity of BER, a natural isoquinoline alkaloid with known anticancer properties.

Enhancing emotion expression (3E): A novel virtual reality tool for improving alexithymia and its psychological outcomes from a multi-dimensional perspective.

Background: Alexithymia is characterized by an impaired ability in emotion awareness, expression, and regulation, associated with deficits in social and mental functioning. This study aims to develop a tool for addressing alexithymia and its psychological outcomes.

Method: In this paper, 102 subjects were recruited to investigate the relationship between alexithymia and psychological outcomes, along with the mediating role of emotion awareness, expression, and regulation.

Assessment of silver-copper co-loaded mesoporous bioactive glass as an advanced pulp-capping material.

Objectives: To evaluate the multifunctionality of silver-copper co-loaded mesoporous bioactive glass (MBG), with the goal of developing an advanced pulp-capping material.

Methods: The synthesis of materials was conducted using the sol-gel method, following the approach described in previous studies but with some modifications. The composition included 80 mol% SiO₂, 15 mol% CaO, and 5 mol% P₂O₅, with additional components of 5 mol% silver, 5 mol% copper, or 1 mol% silver combined with 4 mol% copper, designated as Ag5/80S, Cu5/80S, or Ag1Cu4/80S, respectively.

Extended Reality Technology for Alleviating Adult Dental Anxiety: A Systematic Review and Meta-analysis of Randomized Controlled Trials.

Objectives: To assess the effectiveness of extended reality (XR) technology in reducing dental anxiety in adults, through a systematic review and meta-analysis.

Methods: Comprehensive electronic and manual searches were conducted to identify randomized controlled trials published up to November 23, 2024, with no restrictions on publication year or language. Key outcomes included changes in anxiety and pain levels and cardiovascular indicators, such as blood pressure, heart rate, and oxygen saturation (SpO2).

In-depth site-specific glycoproteomic analysis reveals ER-resident protein PDI regulating wheat yellow mosaic virus infection.

N-glycosylation is crucial in the process of wheat yellow mosaic virus (WYMV) infection, but changes in site-specific N-glycosylation of proteins during WYMV infection have not been well studied. In this study, we employed an intact glycopeptide approach to analyze mock- and WYMV-infected wheat plants. We found that most glycoproteins have N-glycans containing paucimannose or complex/hybrid chains.

The resistance mechanism of B_P225F and B_H272R mutations in succinate dehydrogenase in Botrytis cinerea.

Botrytis cinerea populations resistant to succinate dehydrogenase inhibitors (SDHIs) represent a major problem for the sustainable development of modern agriculture. In the present study, the resistance mechanism of B_P225F and B_H272R mutations in B. cinerea SDH (BcSDH) resistant to SDHIs fungicides, including boscalid (BOS), penflufen (PEN), pydiflumetofen (PYD), fluopyram (FLU), and benzovindiflupyr (BEN), was uncovered.

Genome-wide identification of the papaya-like cysteine protease family in poplar and determination of the functional role of PeRD19A in conferring salt tolerance.

Papain-like cysteine proteases (PLCPs) are a large class of proteolytic enzymes involved in plant growth and development as well as plant responses to biological and abiotic stresses. However, there is no detailed characterization of PLCPs genes in poplar. In this study, a genome-wide analysis of the poplar PtrPLCPs family revealed 47 PtrPLCPs, which were classified into nine subfamilies according to their phylogeny: RD21, CEP, XCP, XBCP3, SAG12, RD19 (5), ALP, CTB, and the lost THI subgroups.

Design of minibinder proteins specific to TNFR1.

TNFα inhibitors have been successfully developed and used in the clinical treatment of autoimmune disorders. However, the use of TNFα blockade may be accompanied by side effects. The cases of bacterial and viral infections, lymphoproliferative disorders, and anti-TNFα-induced lupus, have been reported among the rheumatoid arthritis or Crohn's disease patients treated with TNFα blockers.

Novel and efficient yeast-based strategies for subunit vaccine delivery against COVID-19.

Yeast shows promise as a delivery system for drugs and vaccines due to its specific targeting and immunogenic properties. The objective of this research is to create novel and effective yeast-based methods for delivering subunit vaccines. Through the modification of yeast expression plasmids and optimization of expression techniques, a new dual-expression system has been developed.

Development of L-Met functionalized cellulose nanofiber for an efficient removal of Pb from aqueous solution: Characterization, adsorption behavior, DFT calculations and physiochemical mechanism.

Lead-containing wastewater has been a significant challenge in the field of wastewater treatment. Cellulose surface has a large number of active sites, which is conducive to load modification. And amino acids have rich functional groups, which is a good choice for cellulose modification.

TNFAIP3 affects ferroptosis after traumatic brain injury by affecting the deubiquitination and ubiquitination pathways of the HMOX1 protein and ACSL3.

The occurrence and progression of traumatic brain injury involve a complex process. The pathophysiological mechanisms triggered by neuronal damage include various forms of programmed cell death, including ferroptosis. We observed upregulation of TNFAIP3 in mice after traumatic brain injury.

Follicular cytotoxic T cells is dysfunctional in chronic hepatitis B patients with non-alcoholic fatty liver disease.

Background & Aims: Given the impact of nonalcoholic fatty liver disease (NAFLD) on T cell activation and proliferation functions, we aim to explore the heterogeneity of follicular cytotoxic T (Tfc) cells in chronic hepatitis B (CHB) patients with NAFLD.

Methods: 32 healthy controls (HCs), 36 treatment-naïve CHB patients, and 19 treatment-naïve CHB + NAFLD patients were recruited. We employed multicolor flow cytometry to assess the exhausted phenotype and functionality of Tfc cells.

Role of Life's Essential 8 score in mediating socioeconomic status in the incidence of atrial fibrillation and heart failure:a population-based cohort study.

Objective: To assess whether the Life's Essential 8 (LE8) score mediates the association of socioeconomic status (SES) with atrial fibrillation (AF) and heart failure (HF).

Methods: A total of 236,754 participants from the UK Biobank were included. SES was determined based on household income, education attainment, and employment status using latent class analysis.

Synthetic translational coupling system for accurate and predictable polycistronic gene expression control in bacteria.

Precise and predictable genetic elements are required to address various issues, such as suboptimal metabolic flux or imbalanced protein assembly caused by the inadequate control of polycistronic gene expression in bacteria. Here, we devised a synthetic biopart based on the translational coupling to control polycistronic gene expression. This module links the translation of genes within a polycistronic mRNA, maintaining their expression ratios regardless of coding sequences, transcription rate, and upstream gene translation rate.