Publications by authors named "Manuella C Pires"
Article Synopsis
- Recombinant proteins, essential in biopharmaceuticals, face challenges like low production yield, often due to overlooked biomolecular aspects.
- This study emphasizes the importance of the 5' mRNA secondary structure in enhancing translation and protein production, using BL21(DE3) clones to analyze different genetic variants.
- Results indicated that including an N-terminal His-tag increased protein synthesis by 1.5 times, and optimizing the mRNA secondary structure led to higher production rates, suggesting mRNA analysis can improve recombinant protein yields more effectively than traditional methods alone.*
The impact of cultivation strategy on the cost of recombinant protein production is crucial for defining cost-effective bioreactor operation conditions. This paper presents a methodology to estimate and compare cost impacts related to utilities as well as medium composition, using simple design equations and accessible data. Data from batch bioreactor cultures were used as case study involving the production of pneumococcal surface protein A, a soluble recombinant protein, employing BL21(DE3).
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