High resolution melting analysis to genotype the most common variants in the HFE gene.

Background: High resolution melting (HRM) analysis of PCR amplicons was recently introduced as a closed-tube, rapid, and inexpensive method of genotyping. This study evaluated this system as an option for detecting the three most common mutations in the HFE gene (C282Y, H63D, S65C), accounting for the main form of hereditary haemochromatosis.

Methods: Ninety samples, previously screened by direct sequencing, and 27 controls were used.