Publications by authors named "Manuela Giarin"
Article Synopsis
- Immune-mediated glomerulonephritis with a membranous pattern and masked IgG-Kappa deposits (MGMID) is not well understood, especially regarding the nature of its deposits.
- A case study of a 33-year-old woman showed continuous proteinuria and high levels of SSA antibodies, leading to a kidney biopsy that revealed characteristics of MGMID.
- The study provides the first immunohistochemical evidence of Ro52 positivity in kidney tissues, which may contribute to a better understanding of this condition and its association with autoimmune disorders.
Fibrillary glomerulonephritis (FGN) is a rare glomerular disease characterized by a challenging diagnostic workup requiring ultrastructural identification of 20 nm-thick randomly oriented fibrillar deposits. However, the recent introduction of DNAJB9 as a putative diagnostic marker of FGN could thoroughly improve this diagnostic scenario. This study aims to assess the DNAJB9 immunohistochemical expression in a large series of FGN cases and to eventually confirm its role as a diagnostic marker of FGN.
View Article and Find Full Text PDFCaveolin-1 overexpression has previously been reported as a marker of endothelial injury in kidney chronic antibody-mediated rejection (c-ABMR), but conclusive evidence supporting its use for daily diagnostic practice is missing. This study aims to evaluate if Caveolin-1 can be considered an immunohistochemical surrogate marker of c-ABMR. Caveolin-1 expression was analyzed in a selected series of 22 c-ABMR samples and 11 controls.
View Article and Find Full Text PDFAmyloidosis comprises a spectrum of disorders characterized by the extracellular deposition of amorphous material, originating from an abnormal serum protein. The typing of amyloid into its many variants represents a pivotal step for a correct patient management. Several methods are currently used, including mass spectrometry, immunofluorescence, immunohistochemistry, and immunogold labeling.
View Article and Find Full Text PDFBackground: The prognostic value of changes in paraprotein markers after stem cell transplantation is unknown. We evaluated disease response using serum immunofixation (s-IFIX), total kappa and lambda ratio (KLR), and free light chain (FLC) ratio in myeloma patients who underwent autologous or autologous plus allogeneic stem cell transplantation.
Methods: We studied s-IFIX, KLR, and FLC ratio in sera from 203 patients, 3 months after transplantation.