Publications by authors named "Manuela Garcia-Canovas"
Article Synopsis
- Pig production is under pressure to improve quality, efficiency, and sustainability through advancements in breeding and biotechnology, including in vitro embryo production (IVP) and genetic engineering.
- In vitro embryo production could boost global genetic material exchange, enhance breeding programs, and support the creation of genetically modified pigs that improve agricultural traits and aid biomedical research.
- Despite advancements, current IVP systems face challenges regarding embryo development quality; however, techniques like using high-value oocytes and adding extracellular vesicles to culture media show promise for improved success rates in IVP.
This study examined how the vitrification of pig blastocysts using either the superfine open pulled straw (SOPS) or Cryotop method affects the expression profile of embryonic microRNA (miRNA) transcriptomes, as well as its relation to changes in the expression of target genes (TGs). Surgically collected pig blastocysts were vitrified using either the SOPS method (n = 60; 4-6 embryos/device) or the Cryotop system (n = 60; 20 embryos/device). Embryos were cultured in vitro for 24 h after warming.
View Article and Find Full Text PDFThe most commonly used technique to vitrify pig embryos is the super open pulled straw (SOPS), where a maximum of 6 embryos can be vitrified simultaneously per device without compromising the minimum volume necessary for optimal preservation. Since optimal embryo transfer (ET) demands a transfer of 20-40 embryos per recipient, the customary use of SOPS complicates embryo warming and ET in field conditions. Such complications could be avoided when using the Cryotop® (OC) system, which has been proven to be an effective option for vitrifying at least 20 porcine embryos simultaneously.
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