Publications by authors named "Manuela Federica Scaffino"
Trabecular Titanium (TT) is an innovative highly porous structure that imitates the morphology of trabecular bone with good mechanical properties. Adipose-derived stem cells are a multipotent cell population that can be used in regenerative medicine, in particular, for bone therapeutic applications. The ability of TT to induce the osteogenic differentiation of human adipose derived stem cells (hASCs) in the absence of osteogenic factors was evaluated using molecular biological, biochemical, and immunohistochemical methods.
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- The research focuses on human adipose tissue-derived stem cells (hASCs), which can differentiate into various cell types and have potential in regenerative medicine.
- The study evaluated how these stem cells adhere and differentiate when grown on trabecular titanium scaffolds using biochemical and morphological methods.
- Results indicated that hASCs successfully adhered to the scaffolds, proliferated, developed an osteoblastic-like phenotype, and produced a calcified extracellular matrix, suggesting their effectiveness in bone tissue regeneration.
The mechanism whereby extracellular Ca(2+) exerts the endothelium-dependent control of vascular tone is still unclear. In this study, we assessed whether cardiac microvascular endothelial cells (CMEC) express a functional extracellular Ca(2+)-sensing receptor (CaSR) using a variety of techniques. CaSR mRNA was detected using RT-PCR, and CaSR protein was identified by immunocytochemical analysis.
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