Publications by authors named "Manuel Rui Alves"

Article Synopsis
  • A study aimed at reducing bacteria in goat's milk used pulsed electric fields (PEFs) and heat treatment to process the milk before making cheese, comparing it to cheese made with heat treatment alone.
  • The analysis found no major differences in the overall variety and abundance of bacterial species between the two cheese types, but the PEF-treated cheese showed less microbial diversity and uneven species distribution.
  • Physicochemical properties and sensory scores showed slight variations, particularly in color parameters, with PEF-treated cheese being whiter and more yellow, whereas texture analysis indicated that PEF and heat-treated cheese was less firm and cohesive over time.
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Goat milk is an interesting product from a nutritional and health standpoint, although its physico-chemical composition presents some technological challenges, mainly for being less stable than cow's milk at high temperatures. As pasteurization and ultra-high temperature processing are universally employed to ensure milk quality and safety, non-thermal methods, such as pulsed electric fields (PEFs), reduce the microbial load and eliminate pathogens, representing an interesting alternative for processing this product. This study demonstrates how the combined use of a PEF with short thermal processing and moderate temperature can be effective and energy-efficient in goat milk processing.

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The present work focuses on the detection of SARS-CoV-2 in saliva, contributing to understanding the inhibition effect of the matrix and its influence on the results. Detection of viral genes ORF1ab, N, and E was performed by RT-PCR using saliva directly in the reaction without RNA extraction. Different amounts of saliva were spiked with increasing amounts of viral RNA from COVID-19 patients and subjected to RT-PCR detection.

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The influence of modified atmosphere packaging (MAP, 10% O and 45% CO) on the quality characteristics of fresh-cut green, red and yellow bell peppers ( L. var ) was investigated. Packaging film bags (Krehalon MLF40-PA/PE) with fresh-cut bell peppers were stored for up to 17 days at 5 °C.

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