Cellular uptake mechanisms of an antitumor ruthenium compound: the endosomal/lysosomal system as a target for anticancer metal-based drugs.

Previous studies have described promising antitumor activity of an organometallic Ru(II) complex, η⁵-cyclopentadienyl(2,2'-bipyridyl)(triphenylphosphane) Ruthenium(II) triflate ([η⁵-C₅H₅)Ru(2,2'-bipyridyl)(PPh₃)][CF₃SO₃]) herein designated as TM34. Its broad spectrum of activity against a panel of human tumor cell lines and high antiproliferative efficiency prompted us to focus on its mode of action. We present herein results obtained with two human tumor cell lines A2780 and MDAMB231 on the compound distribution within the cell, the mechanism of its activity, and its cellular targets.

ACP1 genotype, glutathione reductase activity, and riboflavin uptake affect cardiovascular risk in the obese.

Erythrocyte acid phosphatase (ACP locus 1), also known as low-molecular-weight protein tyrosine phosphatase, has previously been associated to glycemia, dyslipidemia, and obesity. In this study, ACP1 genotype and activity were tested in 318 women aged 19 to 83 (mean, 51.74 +/- 13.

Heart rate and blood pressure in mitral valve prolapse patients: divergent effects of long-term propranolol therapy and correlations with catecholamines.

Objective: There is a well known association between mitral valve prolapse (MVP) and low blood pressure (BP), although patients often have high levels of catecholamines and high heart rate (HR). The main objective of our study was to evaluate the effects of long-term adrenergic beta-blockade on these parameters.

Methods: The study population consisted of 46 patients with MVP and the control group consisted of 20 normal individuals.

Activity of two enzymes associated with apoptosis and cell aging in arterial hypertension.

Objective: To study the activity of two enzymes, transmembrane reductase (TMR) and cytosolic low molecular weight protein tyrosine phosphatase (ACP1), in arterial hypertension (HT) in a sample of adults aged over 50 years.

Methods: A sample of 153 adults, 77 with hypertension and 76 normotensive (NT), aged between 50 and 90 years (mean: 71.87 +/- 11.

[Erythrocytic acid phosphatase genetic polymorphism and cardiovascular risk in health children and adolescents].

Objective: To correlate, in a sample of healthy children and adolescents, the activity of the enzyme acid phosphatase (ACP) with its different genetic phenotypes and of these with some cardiovascular risk parameters such as body mass index (BMI), percentage of total fat mass (%TFM), trunk fat (TF), insulin resistance, and the arterial blood pressure (BP).

Design And Methods: The sample was composed of 173 healthy children and adolescents, 96 (55.5%) F and 77 (44.