Development of a microplate-based, electrophoretic fluorescent protein kinase a assay: comparison with filter-binding and fluorescence polarization assay formats.

A microplate-based electrophoretic assay has been developed for the serine/threonine kinase protein kinase A (PKA). The ElectroCapture PKA assay developed uses a positively charged, lissamine-rhodamine-labeled kemptide peptide substrate for the kinase reaction and Nanogen's ElectroCapture HTS Workstation and 384-well laminated membrane plates to electrophoretically separate the negatively charged phosphorylated peptide product from the kinase reaction mix. After the electrophoretic separation, the amount of rhodamine-labeled phosphopeptide product was quantified using a Tecan Ultra384 fluorescence reader.

Disappearing signs of acute myocardial infarction in a patient with viral myocarditis.

A case of acute viral myocarditis with the rapid appearance and disappearance of clinical, laboratory, electrocardiographic, and vectorcardiographic signs of acute myocardial infarction is described in this report. Although segmentary alterations in contractility were demonstrated by ventriculography, coronary angiography revealed normal coronary arteries.