Does thrombopoiesis in multiple myeloma patients depend on the stage of the disease?

Purpose: Infiltration of the bone marrow by neoplastic plasmocytes in multiple myeloma (MM) patients might impair megakaryocytopoiesis. The aim of the study was to evaluate stage-dependent platelet count (PLT) and thrombopoietin (TPO) concentration in comparison to the control group. We also wanted to establish whether TPO might be recognized as a marker of the stage of the disease.

Bone marrow megakaryocytes, soluble P-selectin and thrombopoietic cytokines in multiple myeloma patients.

The expression of adhesion molecules and other cell-surface molecules is substantial in the communication between plasma cells and bone marrow microenvironment, and may lead to increased proliferation of myeloma cells. Many of the cytokines involved in multiple myeloma (MM) pathogenesis, e.g.

Expression of P-selectin (CD62P) on platelets after thrombin and ADP in hypotrophic and healthy, full-term newborns.

Background: Hypotrophic newborns in comparison with eutrophic newborns demonstrate a reduced blood platelet count and therefore may have haemostasis disorders. Expression of P-selectin (CD62P) on the surface of platelets is a marker of stimulated, activated blood platelets. The ability of platelets to react can be determined after the addition of the following activators: strong (thrombin) and weak (ADP).

The evaluation of angiogenesis and matrix metalloproteinase-2 secretion in bone marrow of multiple myeloma patients before and after the treatment.

Purpose: Angiogenesis appears to be a prominent feature of many hematological disorders, particularly in multiple myeloma (MM). Progression in MM also involves secretion of the metaloproteinases (MMPs). In this study, the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and its receptor, in bone marrow trephine biopsy (TB) of thirty six MM patients before and after the treatment or during progression was examined.

Multiplexing analysis of the polyspecific intrathecal immune response in multiple sclerosis.

Intrathecal synthesis of the antibodies specific to neurotrofic viruses: measles (M), rubella (R), Varicella-Zoster (Z), and/or H. simplex (H), known as "MRZH-reaction" plays important diagnostic role in multiple sclerosis (MS). Whereas the analysis of the oligoclonal IgG bands provides high sensitivity, the MRZH-reaction shows high specificity, and hence these methods complement each other.

Platelet expression of CD62P in hypotrophic newborns.

Background: Hypotrophic newborns demonstrate a reduced blood platelet count and therefore may have haemostasis disorders. The antigen density of CD62P on the surface of platelets may indicate the activation of blood platelets.

Materials And Methods: We have studied 31 hypotrophic newborns, and have divided these into two groups: weighing less than the 5th centile and between the 5th and 10th centiles.

Study of the mechanisms of aldosterone prothrombotic effect in rats.

Introduction: We investigated the role of primary haemostasis, fibrinolysis, nitric oxide (NO) and oxidative stress as well as mineralocorticoid receptors (MR) in acute aldosterone prothrombotic action.

Materials And Methods: Venous thrombosis was induced by stasis in Wistar rats. Aldosterone (ALDO; 10, 30, 100 µg/kg/h) was infused for 1 h.

Cerebrospinal fluid leakage--reliable diagnostic methods.

Prompt diagnosis and early treatment of cerebrospinal fluid (CSF) leakage minimizes the risk of severe complications. In patients presenting with clear fluid nasal discharge it is important to identify the nature of the rhinorrhea. The CSF leakage may occur as post-traumatic, iatrogenic, spontaneous or idiopathic rhinorrhea.

[Prothrombotic aldosterone action--a new side of the hormone].

Recent studies have focused on a new wave of interest in aldosterone due mainly to its growing profile as a local messenger in pathology of the cardiovascular system, rather than its hormonal action. In the last few years strong evidence for a correlation between raised aldosterone level and haemostasis disturbances leading to increased risk of cardiovascular events has been provided. It has been demonstrated that aldosterone contributes to endothelial dysfunction, fibrinolytic disorders and oxidative stress augmentation.

[A new look at platelets and microparticles, including their role in haemostatic disorders and progression of neoplastic disease].

Platelets (PLT) are the smallest yet highly reactive components of the circulatory system. Microvesicles (platelet-derived microvesicles - PMV), also known as microparticles or microplatelets (platelet-derived microparticles - PMP), are released from platelets upon stimulation by thrombin, collagen or others platelet agonists. Both PLT and PMP play a role in haemostasis and mediate signal transmission between cells, especially cancer cells, thus modulating their functions.

[Platelets haemostatic in patients with multiple myeloma].

Multiple myeloma (MM) is a type of disseminated cancer which derive plasma cells that are immune system cells in bone marrow and produce monoclonal protein. It represents approximately 1% of all cancers, but recent statistics indicate both increasing incidence and earlier age of onset. Haemostatic disorders are an important clinical problem in patients with MM.

Thrombopoiesis in small for gestational age newborns.

Thrombocytopenia in small for gestational age (SGA) newborns may be due to placental vascular pathology, fetal consumptive coagulopathy and platelet destruction, local imbalance of thromboxane A2 causing placental vasoconstriction and platelet aggregation. Thrombopoiesis in SGA newborns is poorly recognized. In 61 SGA newborns we evaluated thrombocytopoiesis in relation to gender and the rate maturity expressed as <5th percentile and <10th percentile.

Serum sICAM, sVCAM and sE-selectin levels in colorectal cancer patients.

Colorectal cancer (CRC) is one of the most common cancers of the gastrointestinal tract and the fourth cause of cancers death in the world. Soluble adhesion molecules (CAMs) are thought to have an important role in host defense against carcinogenesis. They are biomarkers of inflammation and indicators of the immune response to tumors.

Changes in PDGF concentration in surgically treated colorectal carcinoma.

Purpose: The aim of the present study was to asses the effect of tumor advancement, differentiation grade and surgery treatment on PDGF- AB level and platelet (PLT) count depending on the site of blood collection.

Material And Methods: The study included 38 patients submitted to surgical treatment due to diagnosis of colorectal cancer (CRC) without remote metastasis: G2- 20 patients and group G3- 18 patients. The control group consisted of 24 healthy subjects.

[Cell adhesion molecules and their participation in the process of inflammation and cancerogenesis].

Cell adhesion molecules (CAMs) determine the integral part of protein of cell membranes. CAMs mediate in the reception and the transformation of the information from the external environment. Then CAMs mediate in the transfer of the information in the from of the signal to each fine structure.

[Platelet- derived growth factor--the construction, role and it's receptors].

CONSTRUCTION: The platelet- derived growth factor (PDGF) is a small protein which is produced by many cells. PDGF was originally identified in platelets and in serum. It is a dimeric molecule consisting of disulfide- bonded, structurally similar A- and B- polypeptide chains.

Evaluation of PDGF-AB and sP-selectin concentrations in relation to platelet count in patients with colorectal cancer before and after surgical treatment.

Introduction: Platelet-derived growth factor (PDGF) and P-selectin, the low-molecular weight proteins located mainly in the platelet alpha-granules, are considered to be biologically active markers of platelet (PLT) activation.

Objectives: The study objective was to assess levels of PDGF-AB and sP-selectin in relation to PLT blood count in patients with colorectal cancer (CRC) who were examined before and after radical surgical treatment of the cancer.

Patients And Methods: The study involved 38 CRC patients including B1 - 20 patients (T(2-3)N(1)M(0)), B2 -18 patients (T(2-3)N2M0) and 24 age and sex-matched healthy subjects (the control group).

Megakaryocytes and platelets in experimentally induced renovascular hypertension (2K1C) in rats.

Introduction: Hypertension is one of the most frequently occurring diseases worldwide. Approximately 10% of the population with hypertension reveals the secondary type of the disease. The aim of the study was to evaluate the megakaryocyte-platelet system in the course of renovascular hypertension.

The effect of gestational age on platelet surface expression of CD62P in preterm newborns.

Hemostasis in preterm newborns is characterized by low reserve functional capacity with special reference to the presence of such risk factors as asphyxia or infection. Platelets play vitally important role in hemostasis. Expression of CD62P is a marker of stimulated or activated blood platelets.

Platelet-derived microparticles and platelet count in preterm newborns.

Objective: Does formation of platelet-derived microparticles correspond to platelet activation?

Methods: The study was performed in 51 preterm newborns, 25 girls and 26 boys. The control group consisted of 55 term newborns, 25 girls and 30 boys. Blood samples were collected from the umbilical artery.

[Early diagnosis of bladder carcinoma].

Asymptomatic erythrocyturia is an early symptom of urinary tracts and kidney diseases, including bladder carcinoma. The aim of the research was to compare the diagnostic validity of the cytological urine analysis and the DNA flow cytometry in detecting cancer cells in urine and bladder washings, taken from patients with asymptomatic erythrocyturia, as an early symptom of the bladder carcinoma in situ. The research was conducted on a group of 48 patients (32 male, 16 female, aged 28-55) with asymptomatic erythrocyturia, caused, in 16 cases, by bladder carcinoma in situ, in 18 cases, by bladder carcinoma in situ with urinary tracts infection, and in 14 cases, by the infection alone.

Does prematurity affect thrombocytopoiesis?

Data concerning thrombocytopoiesis in newborns are poorly recognized. Platelets have a crucial role in hemostatic physiology, which is deficient in newborns, especially in preterm newborns. A total of 51 preterm newborns (PTN), 25 girls and 26 boys, were recruited for the study.

[SP-selectin and beta-TG as serum markers of platelet activation in menopausal women with depression].

Unlabelled: During activation, blood platelets (PLT) release a number of micromolecular compounds, of which P-selectin and beta-thromboglobulin (beta-TG) are considered the major markers of the activation. The activated platelets and the released micromolecular compounds actively participate in thromboembolic disorders frequently observed in menopause. Low estrogen level in menopausal women is a common cause of depressive disorders.

[Cerebrospinal rhinorrhea--etiology, clinical signs and laboratory diagnosis].

Last studies have shown unsatisfactory diagnosis of cerebrospinal rhinorrhea. Although the majority of cerebrospinal (CSF) fistulas in the anterior skuli base are traumatic in nature, the minority is non-traumatic or primary. The authors have made an attempt of presenting on the basis of scientific reports of the physiopathology, imagin and diagnosis of cerebrospinal fluid leaks.

[Does colorectal cancer clinical advancement and surgical treatment affect platelet factor 4 concentration].

Unlabelled: The aim of the present study was to determine platelet factor 4 (PF 4) concentration, as a marker of platelet activation, in patients with colorectal cancer (CRC), depending on the clinical advancement of carcinoma and surgical treatment.

Material And Methods: We investigated 21 patients with CRC before the surgery (A0) and three days (A1) and twelve days (A2) after the surgery. The patients were divided into three groups, according the clinical advancement--I, II, III (Hutter's classification).