Draft genome sequence of field isolate Brucella melitensis strain 2007BM/1 from India.

Objectives: Brucellosis is among one of the most widespread important global zoonotic diseases that is endemic in many parts of India. Brucella melitensis is supposed to be the most pathogenic species for humans. Here we report the draft genome sequence of B.

Resurgence of diphtheria in rural areas of North Karnataka, India.

Background: A diphtheria outbreak was identified from Vijayapura (formerly Bijapur) district in the South Indian state of Karnataka in 2011. There was a surge in the number of throat swab samples received under the Integrated Disease Surveillance Programme (IDSP) in North Karnataka since then.

Objectives: A microbiological study was undertaken to generate information on the status of resurgence of the disease in the region.

A rare case of mycetoma due to curvularia.

Mycetoma due to Curvularia is a rare clinical entity. Here, we report a case of 27-year-old female presented with multiple swellings and discharging wounds around left shoulder joint since 12 years. Local examination showed diffuse nodular swellings over left anterior chest wall, posterior chest wall, and axilla.

Multidrug-Resistant Vibrio cholerae O1 was Responsible for a Cholera Outbreak in 2013 in Bagalkot, North Karnataka.

Cholera is a major cause of illness in the developing world. During the monsoon season, small sporadic clusters of cholera cases are reported on an annual basis in Karnataka, India. During the monsoons of 2013, there was a cholera outbreak in Badami, a remote area of Bagalkot district in Karnataka.

Twin outbreak of cholera in rural North Karnataka, India.

Background & Objectives: Successive outbreaks of acute watery diarrhoea occurred in Talikoti and Harnal, located in Bijapur District of the southern Indian s0 tate of Karnataka, in July and August 2012, respectively. These outbreaks were investigated to identify the aetiology and epidemiology.

Methods: Information was collected from the local population and health centres.

Clinical utility of a quantitative Rose Bengal slide agglutination test in the diagnosis of human brucellosis in an endemic region.

Background: Brucellosis currently ranks as the most important zoonotic disease in the world. Brucellosis is difficult to diagnose because patients often have nonspecific clinical symptoms that can be attributed to a number of disease agents prevalent in the area. Thus, this has necessitated the dependency of clinicians on microbiological confirmation, very often by sero diagnostic methods.

Multidrug-resistant Vibrio cholerae O1 in Belgaum, south India.

An outbreak of acute diarrhoea occurred in the Belgundi area (population 3896) of Belgaum Taluka (population 815 581) in Karnataka, South India, in June 2010. An estimated 16.22 % of people were affected and 0.

Comparison of a novel immunocapture assay with standard serological methods in the diagnosis of brucellosis.

Background: Microbiological culture methods and immunological assays currently available are technically challenging, difficult to interpret even in non-endemic areas. They are also time consuming leading to misdiagnosis, treatment delay, and severe morbidity and mortality. Therefore, the development of a simple and accurate diagnostic assay which could be performed even in small laboratories is a pressing need.

Splenic Abscess due to Brucella Melitensis - A Rare Pediatric Complication.

Splenic abscess due to Brucella species is an extremely rare complication especially in acute illness. Here we report a case of splenic abscess caused by Brucella melitensis biotype 1 in a child with acute infection who was successfully treated with only antibiotics.

A fatal case of empyema thoracis by Nocardia farcinica in an immunocompromised patient.

Empyema thoracis by Nocardia farcinica infection is uncommon. Here we report a rare and fatal infection in a 27-year-old HIV- seropositive male who presented with cough, expectoration, and breathlessness. Nocardia farcinica was isolated from sputum and pus from the pleural cavity.

ELISA versus conventional methods of diagnosing endemic brucellosis.

The diagnostic value of enzyme-linked immunosorbent assay (ELISA) was evaluated when blood specimens of 92 patients suspected of brucellosis underwent the ELISA (IgM and IgG), standard tube agglutination (SAT), and 2-mercaptoethanol (2-ME) tests and blood cultures; 38 sera from non-brucellosis patients and 34 sera from blood donors were also subjected to ELISA, SAT, and 2-ME tests. SAT was able to pinpoint only 23 (25%), whereas ELISA confirmed the etiology in 56 (60.9%; P < 0.

Rickettsial meningoencephalitis in a child--a case report.

Severe central nervous system involvement has been reported in adults with Rickettsia conorii infection but rarely in children. We report here a serologically documented case of meningoencephalitis in a child caused by spotted group R. conorii.

Brucellosis in India - a review.

Brucellosis is an important re-emerging zoonosis with a worldwide distribution. It is still an uncontrolled serious public health problem in many developing countries including India. Brucellosis in India is yet a very common but often neglected disease.

Meningitis due to Rhodotorula glutinis in an HIV infected patient.

Rhodotorula spp, though considered a common saprophyte, recently has been reported as causative agent of opportunistic mycoses. We present a case of meningitis in an immunocompromised human immunodeficiency virus infected patient who presented with longstanding fever. He was diagnosed as a case of chronic meningitis.

Brucellar meningitis in an infant--evidence for human breast milk transmission.

Brucellar meningitis is relatively uncommon, especially in children younger than 1 year. We present another case of meningitis due to Brucella melitensis biotype 1 in a 11-month-old infant transmitted by breast milk. This is the first report of successful isolation of B.

Bacteremia is as unpredictable as clinical manifestations in human brucellosis.

Objectives: Because of the suboptimal recovery rate of brucellae from blood, it has been proposed that cultures of bone marrow, liver tissue, and lymph nodes may improve the recovery rate of the organism. Data in support of these recommendations are limited and not clearly convincing, especially that of bone marrow culture. The main purpose of this work was to evaluate the roles of blood, bone marrow, liver, and lymph node cultures in the diagnosis of human brucellosis.

Review of clinical and laboratory features of human brucellosis.

Infection with Brucella spp. continues to pose a human health risk globally despite strides in eradicating the disease from domestic animals. Brucellosis has been an emerging disease since the discovery of Brucella melitensis by Sir David Bruce in 1887.

Brucellar epididymoorchitis - report of five cases.

We report here 5 bacteriologically proven cases of Brucellar epididymoorchitis. Four cases presented with unilateral epididymoorchitis and with bilateral presentation in one case. Blood culture grew Brucella melitensis in all 5 cases.

Keratomycosis due to Exserohilum rostratum - a case report.

We report here a case of keratomycosis by Exserohilum rostratum. An 18-year old female patient presented with pain and watery discharge from the left eye since 10 days. Clinically the case was diagnosed as keratomycosis.

Diagnostic yield of blood clot culture in the accurate diagnosis of enteric fever and human brucellosis.

Culture of blood is the most frequent, accurate means of diagnosing bacteremia in enteric fever and brucellosis. However, conventional blood culturing is slow in isolating bacteria causing these diseases. In this work, we evaluated the performance of blood clot culture and conventional whole blood cultures in the accurate diagnosis of enteric fever (253 cases) and human brucellosis (71cases).

Protean clinical manifestations and diagnostic challenges of human brucellosis in adults: 16 years' experience in an endemic area.

A prospective study was carried out to elucidate the clinical, epidemiological and laboratory features of human brucellosis. A total of 26 948 blood samples (from adults aged 15 years and above) were screened for serological evidence of brucellosis over a period of 16 years. The slide agglutination/Rose Bengal plate agglutination test gave positive results in 517 patients, of which 509 had detectable titres by the standard tube agglutination test (SAT).